Is this wild yeast or bacteria?

[Gentlemans_Ale]

I need an expert to help me identify these colonies as wild yeast or bacteria. This is an attempt to isolate wild yeast from plants in my garden. Besides the mold contaminants, there are clusters of round, pinkish-white colonies. I suspect bacteria but hopeful I isolated some wild yeast. Any microbiologists out there? I don’t have access to a microscope.

 

[TheMadKing]

What is the agar that you used?

 

[TheMadKing]

Usually yeast isn't pink.. Could be a methylobacterium, normally the only common pink colony from dirt.

 

[Gentlemans_Ale]

Malt extract

 

[Gentlemans_Ale]

I think you nailed it

 

[hopjuice_71]

Sometimes Serratia marcesens looks pink. Also quite common in the soil, but something you don't want to intentionally deal with.

 

[RPh_Guy]

What did you streak onto the plate?

Try mixing some whatever into some wort and incubate for a few days. If it ferments, then you have yeast. Streak a sample of liquid at that point.

Hope this helps

 

[Gentlemans_Ale]

What did you streak onto the plate?

Try mixing some whatever into some wort and incubate for a few days. If it ferments, then you have yeast. Streak a sample of liquid at that point.

Hope this helps

I just streaked directly from plant leaves to plate using a sterile swab. Thanks for the tips, helps very much!

 

[Vale71]

If you want to isolate wild yeast I would suggest culturing anaerobically. That way you will screen out a lot of unwanted bacteria and molds as well. It will just take a few more days to get a result as anaerobic growth is obviously slower.