Hello everyone,
I am looking for some help identifying some sort of fungi that I have found in one of my attempts at isolating a wild yeast. I am a chemist, not a microbiologist and need some help!
The back story is around christmass I bought myself a scope as a present to myself, and ever since have been looking at just about every thing I can find.
One day while brewing I pulled a nice smelling flower from the garden and placed it into 12ml of sterilized YEPD media with 5% glucose. Fermentation was evident in roughly 48 hours, but I let it sit for 2 weeks venting it daily.
Anyway I finaly decided to look at a drop under the scope. I stained with methylen blue and took a look. At 400x there are what appear to be yeasts, large round cells that are darkly stained blue. However these yeasts are showing multilateral budding. Most common was a single large cells with 4 smaller daughter cells fused to the mother cell. They almost look like a tetrahedron, with the mother cells being the tip with the daughter cells aranged equidistant from each other.
The other peculiar thing is this fungus seems to form pseudohypha, but I am not really qualified to make this claim. They form a very irregular chain of fused cells.
Anyway I streaked some of this solution out yesterday in order to better see what is in there.
But I would really appreciate if anyone versed in the subject would chime in.
Thanks!
I am looking for some help identifying some sort of fungi that I have found in one of my attempts at isolating a wild yeast. I am a chemist, not a microbiologist and need some help!
The back story is around christmass I bought myself a scope as a present to myself, and ever since have been looking at just about every thing I can find.
One day while brewing I pulled a nice smelling flower from the garden and placed it into 12ml of sterilized YEPD media with 5% glucose. Fermentation was evident in roughly 48 hours, but I let it sit for 2 weeks venting it daily.
Anyway I finaly decided to look at a drop under the scope. I stained with methylen blue and took a look. At 400x there are what appear to be yeasts, large round cells that are darkly stained blue. However these yeasts are showing multilateral budding. Most common was a single large cells with 4 smaller daughter cells fused to the mother cell. They almost look like a tetrahedron, with the mother cells being the tip with the daughter cells aranged equidistant from each other.
The other peculiar thing is this fungus seems to form pseudohypha, but I am not really qualified to make this claim. They form a very irregular chain of fused cells.
Anyway I streaked some of this solution out yesterday in order to better see what is in there.
But I would really appreciate if anyone versed in the subject would chime in.
Thanks!
