I used a calculator wich isn't more then a rough guideline IMHO and ik gave me about 90BL units. I decided to dump my last bit DME in (boiled) so I added 1l 1.030.
The plan now is to cool crash wednesday or thursday, For my first brew I was thinking of a simple LME kit 1.058 15L. Then use the yeast cake 3 weeks later for my actual brew. Very time consuming project but a lot of fun too! Thanks a load for your answers. Will update later this week!
As long as you keep swirling that flask you should grow new and healthy cells.
When step starters get a little bigger than say 250-500 ml, the preferred method (the more so if we have ample time before brewing) is to cold crash and decant in between steps, to rid the culture of the old starter beer/old wort mixture (containing alcohol and other waste products). It also prevents (heavy) dilution of the new wort added. Keep that in mind for next time.
I'm curious how it all turns out. Keep us updated!