what about saving *some* of the yeast cake at the bottom of the fermenter in a sterilized container? instead of doing the yeast washing, could you save some of the yeast, trub, break material and all, then add some of that slurry to a later batch at the appropriate pitching rate? based on what you said, overpitching on a whole yeast cake is not optimal and saving some yeast & trub in a second sterilized container will give you an opportunity to clean and sterilize your carboy/fermenter. this sounds like it will work fine and i have done this in the past and it worked fine and dandy.
anything i should be worried about? precautions i should take? qualms on why this is not appropriate? what harm can a tiny bit of trub and break material do anyway?
Yeast harvesting is conducted in precisely this manner daily in breweries and brewpubs all across the globe.
Yes, it's possible.
Take normal cleanliness and sanitation precautions. Make sure the implements you use to harvest and store your slurry are scrupulously clean and completely sanitized. I always use steel implements to harvest (using caution not to ding the walls of the plastic vessel) and store in glass (Mason jars).
Refrigerate your harvested slurry immediately upon harvesting.
LABEL THE SLURRY JAR.
Learn what's good slurry and what isn't. Good yeast comes from the middle layers - the gunk in the bottom of the vessel is stratified - and smells/tastes like yummy beer yeast. Don't be afraid to taste it.
LABEL THE SLURRY JAR.
Don't be afraid to just mix up the whole damn mess and harvest it. That will ensure the yeast is stored under a layer of beer - the best possible situation - in the storage vessel. If you do that, make sure you set the bottom indicator in the Mr Malty Calculator to max (25%).
LABEL THE SLURRY JAR.
If you're going to store the slurry longer than a week or so, start following the yeast-washing steps for long-term storage. It won't hurt to wash your harvested yeast anyway, though it's not strictly necessary; if you're interested in the practice, why not try it?
Oh, and LABEL THE SLURRY JAR.
If I go by Ray Daniels numbers in Designing great beers i come up with over a Trillion yeast cells in 1qt of yeast cake. His charts says there are 1.5 billion yeast cells per ML of yeast slurry, and there are 946Ml in a quart. That comes out to 1.4 Trillion cells in a quart. Does that sound right?
Yes and no. Different strains have different cell sizes. Weight and volume are only a rules of thumb, not hard and fast. 1 ml of 1076 might have 12 billion cells in it. 1 ml of 2007 might have 8 billion. Moreover, the density of the slurry has a lot to do with it. A thin, runny slurry is going to have fewer cells than a dense slurry.
Daniels's numbers are pretty much on. It's close enough to work well. The only way you're going to get more accurate than that is if you conduct your own viability tests in your own lab.
Also, what really defines slurry? Yeast cake with all the trub, or washed yeast?
Yes.

Washed yeast is still not the same thing as cultured yeast straight from the smack-pack, so the same rules apply. Washed yeast is a certain amount more "dense" than non-washed yeast, in terms of cell count, because washed yeast has had most if not all of the non-yeast matter removed from the slurry. But there's no way to establish an across-the-board number of how much, because that changes in every brewery and every strain.
Ah, yes, *that* Bob. We've had this discussion before. I agree to disagree, despite your disparaging remarks about beer you've never tried.
We shall agree to differ, despite my sure and certain knowledge that I've tried beers brewed both ways and can spot the properly-brewed one 85% of the time. I don't mean to say you can't brew beer by just knocking out wort onto a yeast cake. I mean to say you can't
consistently brew
excellent beer as you can by following proper, established brewing procedures.
Cheers, all!
Bob