I usually use about half a teaspoon of irish moss per five gal yield, but that gets filtered out during lautering...would that layer be made of flocculated proteins or were you thinking the proteins plus the plant particulates from the wort or just the settle proteins? Thanks again for your responses.
Irish moss isn't filtered out during lautering, as lautering happens in the mash/lauter tun.
I think you mean that you filter the wort after it's cool? If so, there is no need to do that but you can if you want.
Cold break looks like big globs of goobers (for lack of a better description) like this:
Hot break is finer and smaller, and looks like egg drop soup in your wort.
Irish moss (or whirlfloc) help in the boil by coagulating the small protein particles so they are heavier, and so gravity has them fall out. You can filter it out if you want, but if you don't, it will fall to the bottom with the trub and compact down a lot.
I have counterflow chiller, so all of the cold break ends up in the fermenter. Some brewers prefer to filter it out, but it really doesn't matter in the end either way.
Edited to say that these are NOT my photos! I took them from an image search on the internet, and this is attributed to the Northern Brewer forum.