Chicago Homebrewers Yeast Bank Project

[Will-i-am]

**** fools like I say I'm a diagnostic supervisor in a food microbiology lab ... I have access to an autoclave instead of sterilizing at home with a pressure cooker ... I can bring all the supplies needed to slant and I can also provide slants I would be more then happy to teach aseptic technique and proper slanting ... **** is easy if you follow some simple rules as far as what I want ... Just some of the yeast and enough cash to cover supplies if you want some I can sell on the spot or pm me ... Also pm me with any questions and I'll try to get back to you in a timely fashion ... I can and would love to teach anyone how to be successful at slanting just setup a time and place ... Hopefully somewhere on the south side or northwest Indiana but I can travel too just might take a bit o planning to schedule something.

 

[inverted]

I'm interested let me know when

 

[bmason1623]

Can I just add that we are not limited to just slanting? I eventually want to learn how to slant (did you hear that professor wegz?) but in the meantime I will wash yeast. I'm thinking that we start "building" the bank with whatever form we currently use just to build up the variety of yeasts available. However, I will go along with everyone elses suggestions. Again, these are just my initial thoughts. Comments, rebuttals are always welcomed.

 

[wegz15]

Not saying just sticking to slanting. Just throwing suggestions out there.

 

[wegz15]

How many would be interested in getting culture tubes to slant? I might buy a case of 1000 plus 1000 caps. They would be no more than $0.75 a set. Don't need 1000 so if there isn't that much interest I'm not buying them.

 

[bmason1623]

wegz, i'll go in on this buy but for a couple dozen maybe not too many. can you send me the link to where you're thinking of buying from?

 

[bmason1623]

How many would be interested in getting culture tubes to slant? I might buy a case of 1000 plus 1000 caps. They would be no more than $0.75 a set. Don't need 1000 so if there isn't that much interest I'm not buying them.

A set consists of one tube and one cap? If so, then the price per 1000 would be $750.00? That seems pretty steep if that's the case.

 

[Uziyahu]

I'm interested in learning yeast washing or slanting or both, but I wouldn't be up for buy too many supplies without understanding how both processes work and the necessary storage. Storage space is my most limiting factor as it is for many in Chicago.

 

[wegz15]

The 75 cents is a max. Only did a little bit of searching. Not sure how much shipping Is or if there is a cheaper source yet.

http://www.medixcorp.com/catalog/detail.asp?item_id=5830

http://www.amazon.com/gp/product/B0018EJAY4/?tag=skimlinks_replacement-20

 

[bmason1623]

The 75 cents is a max. Only did a little bit of searching. Not sure how much shipping Is or if there is a cheaper source yet.

http://www.medixcorp.com/catalog/detail.asp?item_id=5830

http://www.amazon.com/gp/product/B0018EJAY4/?tag=skimlinks_replacement-20

I can't seem to find how many are in a case in the link that you provided.

 

[bmason1623]

I can't seem to find how many are in a case in the link that you provided.

wait, never mind. A case is 1000 pieces. I must be going blind.

 

[wegz15]

”List Price: $301.18 Medix Price: $235.06 per Case(s) You Save: $66.12 (22%)

Size: 1 Case(s) 1000 per case”

 

[bmason1623]

”List Price: $301.18 Medix Price: $235.06 per Case(s) You Save: $66.12 (22%)

Size: 1 Case(s) 1000 per case”

yep, caught it on my third look at it.

 

[starman]

I'm in to some extent. How many make sense?

 

[inverted]

I'm in for about 25$ worth

 

[wegz15]

Doesn't seem to be much interest.

Anyone need 2038 munich lager?
I'm about to make some slants. I can make extra if anyone wants one, let me know in the next half hour.

 

[bmason1623]

Doesn't seem to be much interest.

Anyone need 2038 munich lager?
I'm about to make some slants. I can make extra if anyone wants one, let me know in the next half hour.

I will take you up on your munich lager slants. I've been busy at work but once it subsides I'll try to get this project moving. Not sure how but I'll try. Maybe a group buy with a prerequisite that you must exchange yeast with others to participate.

 

[wegz15]

Made you a couple of slants. They should be ready in about a week. I still have to use your belgian? yeast you gave me. I need a recipe.

 

[Alexeit]

Hey all,
So I have some Petri dishes with yeast and some culture tubes with yeast and I'm not entirely sure how to fill out this information out. Some of the yeast are straight out of the bag, i.e. I had some left over in the bag post pitching and I just diluted it down with some clean water and placed them on petri dishes and on slants. So in this case are they generation 1 or generation 0? The reason I ask is because most my "slants" are in this format, thought I do have some stored away in liquid form as well.

Also, if anyone is interested in having slants made I might be able to do them for you, as long as you provide the materials. I work in a lab and have access to an autoclave, and some sterile pipettes, as well as glassware and ultrapure water.

 

[Alexeit]

Also, another thing i should mention, although slanting and washing of the yeast is fine, another alternative, which might be even better, is to save them in a glycerol "freezer stock" which will remain viable for a much longer period of time, and which only requires that you scrape some of the freezer stock into a solution and let it go.

 

[Polboy]

I want to say again that i like the idea of the yeast bank but im not sure how to overcome problem of logistic, simply i have a hard time to justify long drive to pick up yeast if i can get them at lhbs for $3.5-6.5 when shopping for other ingredients anyway. In my case it would work for club meetings or during group buy pickups or for strains that are not commercially available (right now im making starter from bottle of orval that i will be freezing) but this doesnt happen too often

 

[RC0032]

Im with Polboy it has to be economical for this to work.

Everyone move to the city

 

[wegz15]

I thought about this as well. I figured I could throw slants in a padded envelope and mail them.

 

[TheBreweryUnderground]

Im with Polboy it has to be economical for this to work.

Everyone move to the city

I just realized that there are people who live outside of Chicago.

 

[Polboy]

im still in the city limits but just on the edge, it took me 30+ min to get to bmason and probably he is the closest.
btw i have jar of 3068, 3787 and in 3-4 weeks (brewing tomorrow i will have 3711 and orval, if you are around midway and want to pick it up let me know

 

[Alexeit]

So I got another question...

I have access to a lyophilizer, and liquid nitrogen, do you guys think lyophilizing yeast will be a good idea? I head that it's possible to lyophilize and then just store in a fridge while it's dry for years.... Also this will make sending them out easier, cuz you know, dry powder and such doesn't weigh too much and you can just drop them into a starter like a dry packet.

 

[Polboy]

i dont know how pros do it but dry yeast are most stable so why dont you give it a try, if you can measure viability do it over time if you cant i can count it with hemocytometer and live/dead dye for yeast so you could send it to me

 

[Alexeit]

sounds like a plan, I will probably lyophilize them tomorrow night, and hopefully I'll do a quick dirty test with sugar water, and if that works I will probably send them to you to test the viability

 

[bmason1623]

Would it be easier for people if we stored/banked the yeast at my place?

 

[Will-i-am]

I can check for viability and also offer counts ... Cfu per gram to see what your innoculm would be .... I work as a food microbiologist and have access to many supplies and toys ... Lyophilization is some cool stuff ... Your probably gonna wanna use a small bit of glycerine to keep from rupturing cell walls tho that will increase viability