Sanitation aside, trying to determine pitch size has always been the big issue for me with using harvested yeast. Most of that is a side-effect of less than compulsive break removal during the brewing process. Which means there's a lot of trub to go along with the post-fermentation yeast collection.
So then I try to relate what I have on hand with various calculators, with pretty much no confidence that there's a correlation
And then there's the whole bottle-dregs resurrection thing. I have this lovely Spencer yeast that I'm now quite certain I had not propagated up to an appropriate pitch size, hence its batch is still puttering along, having finally reached 1.025 - a solid ten points high.
I'm probably going to have to rack this batch off its yeast cake on top of the 3787 cake from the other batch, so I'll still have the Spencer yeast to build up for another try.
Stumbled on
this article from a year ago. Another interesting take on Spencer...
Cheers!