brumer0
Well-Known Member
Howdy all,
I am doing my first petri dish culture from a Chimay bottle. Ive propagated the yeast up to 400ml and now want to get a pure strain by using the petri dish method. I finally was able to make some agar (the first batch was too thick, the second to thin) and poured them into the petri dishes and it solidified well.
I dont have a pressure cooker, and am not planning on buying one for a month or so (if I can get away with it). So far I have used a pot and steamed everything for 15 minutes for sterilization. I put the petri dishes in there, and 2x they have melted. Am I doing something wrong? I am using a big kitchen pot and a metal vegetable steamer to keep it above the water, and just putting the petri dishes on that. Do I need a pressure cooker, and if so, wont these melt in that too?!
Thanks!!
~Brandon
I am doing my first petri dish culture from a Chimay bottle. Ive propagated the yeast up to 400ml and now want to get a pure strain by using the petri dish method. I finally was able to make some agar (the first batch was too thick, the second to thin) and poured them into the petri dishes and it solidified well.
I dont have a pressure cooker, and am not planning on buying one for a month or so (if I can get away with it). So far I have used a pot and steamed everything for 15 minutes for sterilization. I put the petri dishes in there, and 2x they have melted. Am I doing something wrong? I am using a big kitchen pot and a metal vegetable steamer to keep it above the water, and just putting the petri dishes on that. Do I need a pressure cooker, and if so, wont these melt in that too?!
Thanks!!
~Brandon