All - I recently harvested some Conan yeast, stepped it up 4 times before pitching most of it into a clone attempt, then I estimated I was left with about 80 billion cells so I started stepping it up again until I reached ~443 billions cells for another big DIPA. I pitched that second starter with ~ 80 billion cells left and I want to continue stepping this up and using it for a few more beers/ciders...
Anyone have experience doing this? My concern comes from the generally excepted knowledge that you can only reuse yeast (from primary) a few times before it starts to do weird things, but if I am stepping up the yeast in a low gravity, controlled starter where I am not really stressing the yeast too much, can I just continue to step it up (slowly) and not have to worry about the yeast changing too much?
Thanks!
Anyone have experience doing this? My concern comes from the generally excepted knowledge that you can only reuse yeast (from primary) a few times before it starts to do weird things, but if I am stepping up the yeast in a low gravity, controlled starter where I am not really stressing the yeast too much, can I just continue to step it up (slowly) and not have to worry about the yeast changing too much?
Thanks!