I usually start with a lighter/lower hopped beer and after primary fermentation rack that beer into the secondary and then re-harvest what is left by scooping out the sediment from the primary. This includes alot of extras that probably are not "pure" yeast cells. Everything is sanitized -scooper, bottles, hands, etc. Also I try not to breathe much and if I do I exhale away from the area. I scoop into brown plastic (Mr. Beer Bottles-I knew I would find a use for these things!) about 2 cups of the slurry, then squeeze out excess air and cap. Then I throw these in the fridge and release built up pressure regularly(every few days) being careful only to squeeze air out NOT in. Then on brew day I treat these as I would any vial of yeast and pitch into the next batch.
Now this is not the cleanest and most sanitary way, but I have been able to save $ on yeast while experimenting with different grains and hops on new brews. This allows me to get a more pronounced pallete for grains and hops without the extra cost of new yeast. I have not made a funky beer yet. (knock, knock, knock)
After practicing this for the past year I think I will now start to practice "washing" my yeast before pitching. The learning never stops!