If you have been conditioning an ale it at room temps, you should still have enough live yeast in the beer to just be able to add priming sugar for carbonation. There are some ale yeasts, like S-04 for example, that flocculate extremely well and pack into a tight cake, so that when you package it sometimes it doesn't carbonate as quickly, but in my experience it still gets there.
In some cases where you are lagering at lower temps for an extended time, a lot of the yeast will drop out which can cause problems priming. HOWEVER even in that case, you can still add a bit of yeast to your priming solution at packaging time, and that will help things out.