why are you doing two 1 liter steps? you're not going to get a whole lot of growth out of the second one liter. might as well go straight to 1.8, no?
I was going by the numbers I got from yeastcalc.com. I started with an assumption of 2 billion cells from can dregs. Here's what I got from the steps. Maybe I am looking at it the wrong way (my assumptions were using the Jamil Stirplate setting)
1. 2 billion cells into 150ml gave a yield of 11 billion cells
2. 11 billion cells in 400 ml gave a yield of 45 billion
3. 45 billion cells in 1L gave 147 billion cells
4. Started in a new tab with 147 billion cells. 147b in 1 L almost doubled to 274
5. 274b in 1.8 L gave me a final count of 515 billion
If I take out the second 1 L step, I get this:
1. 2 billion cells into 150ml gave a yield of 11 billion cells
2. 11 billion cells in 400 ml gave a yield of 45 billion
3. 45 billion cells in 1L gave 147 billion cells
4. Started in a new tab with 147 billion cells. 147b in 1.8 L yields 359 billion.
Am I missing something? I would think the extra 1L step is worth the extra 150 billion cells. That's more than a standard White Labs vial worth of difference.