optimatored
Well-Known Member
For all who are interested:
In my microbiology class we were asked to bring in samples of "cleaners" from home (Lysol, bleach...). We will be placing them on plates with "lawns" of bacteria to see the effectiveness of random cleaning agents.
My plan is to bring in Star San and Iodophor... if they let me I will take pictures for everyone to see and post the results. This will be in about a week or so.
So to the vote... what will be more effective?
Star San or Iodophor?
Just a few notes on the experiment:
We will be doing a filter paper method to determine effectiveness. We soak a paper disk in the sanitizer and then add the disk to the bacterial plate. The greater the area of "clearing" the more effective the cleaning agent is against the type of bacteria. In this case is either B. subtilis or S. aureus.
Just a simple way to determine effectiveness and more interesting that scientific. As some agents may just travel father on the plate that others.
UPDATE 4/20/2010
The bacteria I was given is S. aureus... We inoculated each plate with our bacteria, then soaked a paper disc in the following.
4 plates total: 1 Star San, 1 Iodophor, 1 Phenol, and 1 is straight bleach. Results will be next week.
RESULTS 4/27/2010
There really is no winner in the end... I am going with inconclusive results because Iodophor really did nothing at all. This could be because the Iodophor evaporated off the disc, did not diffuse across the plate, or my concentration was low. Who knows, but it was fun any way. Just in case I am sticking with Star San!
Note the cleared area around each white disk showing no bacterial growth. Pictures were taken with my cell phone so are not the best.
In my microbiology class we were asked to bring in samples of "cleaners" from home (Lysol, bleach...). We will be placing them on plates with "lawns" of bacteria to see the effectiveness of random cleaning agents.
My plan is to bring in Star San and Iodophor... if they let me I will take pictures for everyone to see and post the results. This will be in about a week or so.
So to the vote... what will be more effective?
Star San or Iodophor?
Just a few notes on the experiment:
We will be doing a filter paper method to determine effectiveness. We soak a paper disk in the sanitizer and then add the disk to the bacterial plate. The greater the area of "clearing" the more effective the cleaning agent is against the type of bacteria. In this case is either B. subtilis or S. aureus.
Just a simple way to determine effectiveness and more interesting that scientific. As some agents may just travel father on the plate that others.
UPDATE 4/20/2010
The bacteria I was given is S. aureus... We inoculated each plate with our bacteria, then soaked a paper disc in the following.
4 plates total: 1 Star San, 1 Iodophor, 1 Phenol, and 1 is straight bleach. Results will be next week.
RESULTS 4/27/2010
There really is no winner in the end... I am going with inconclusive results because Iodophor really did nothing at all. This could be because the Iodophor evaporated off the disc, did not diffuse across the plate, or my concentration was low. Who knows, but it was fun any way. Just in case I am sticking with Star San!
Note the cleared area around each white disk showing no bacterial growth. Pictures were taken with my cell phone so are not the best.