Yeast slant media recipe

In the Slanting Yeast sticky there are two conflicting recipes for the media.

On page one:
400mL of water
35g of DME
2.5g agar agar

On page eight:
500mL of water
7.5 grams of agar
35 grams of DME
5 grams of the nutrient


I would like to know if anyone out there has a recipe that never fails them. Something you have been been able to repeat with success. I have powdered agar agar, so my ideal recipe would use that. Thank you in advance for sharing!

I wasted alot of time and DME trying to get the powdered agar to work with no success. As soon as my agar agar came in from amazon I made up the media using pretty close to the same recipe as on page eight minus the nutrient.

Westel said:

I wasted alot of time and DME trying to get the powdered agar to work with no success. As soon as my agar agar came in from amazon I made up the media using pretty close to the same recipe as on page eight minus the nutrient.

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If powdered agar agar didn't work, what type did?

Here is what I have:

http://www.asiansupermarket365.com/Telephone-Brand-Agar-Agar-Powder-p/htbaayej.htm

I used the recipe on page one and used powdered agar. Worked well, but I did have some fluid in the slant, although I am not concerned as it is sterile. The agar slants are still good to go a month later. I used some this morning, and it is easy to poke the agar media with a inoculation loop.

I used the first one with agar from morebeer and they fell apart, used a recipe similar to the second and I have to boil the slants to get the media out after I make a starter.

sapperxl said:

I used the first one with agar from morebeer and they fell apart, used a recipe similar to the second and I have to boil the slants to get the media out after I make a starter.

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Similar? Would you mind posting the actual recipe?

Here's what I have now http://www.amazon.com/dp/B001M23WR8/?tag=skimlinks_replacement-20

The stuff I couldn't get to work was from midwest not sure why but I could never get it to set up.

Spica66 said:

Similar? Would you mind posting the actual recipe?

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Looked up my notes and it's the exact same recipe as the second, I just doubled it so I could fill more slants, I make about 200 a time since they store almost indefinitely.

sapperxl said:

Looked up my notes and it's the exact same recipe as the second, I just doubled it so I could fill more slants, I make about 200 a time since they store almost indefinitely.

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Next time I need yeast I'm skipping MW and just calling sapper

My recipe I used was 250 ml h20
3.4g agar agar
17.5g DME

Spica66 said:

Taking into account everything I could read on the internet and the responses received from the fine people on this forum, I am going to use the following recipe for attempt #1:

1% = 1g/100ml

7.0% DME
1.5% Agar-agar
1.0% Yeast Nutrient

200ml Tap Water
14g DME
3g Powdered Agar-agar
2g Yeast Nutrient
Red food coloring

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That looks like it'll work just fine but what's the red food coloring for?

I like the contrast the red gives gives between the media and the colonies.

https://www.homebrewtalk.com/f163/slanting-yeast-133103/index15.html#post2286547

I used some Agar flakes from Wholefoods (pricey but I didn't have time to order it). I used the recipe on page 8 and it set up fast and seems like a good balance. I gave a sharp slant (maybe too sharp) and inoculated my first slants and plates last night. That recipe worked well, but time will tell.

I also used the red food coloring, just a small bit, and it came out pink before I pressure cooked it. I think the heat denatured my color, cause it looks like dme mixed agar media, no extra color now.

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The recipe worked perfectly! Thank you all for your help.