Recent content by senaVZ

I would get the cheapest one you can find that's not disposable. They should come with instructions but if they don't, then they're probably the standard counting area and use 10^4 (as mentioned above) times your average cell count. I've used standard coverslips in case you break the one that...

I've seen something similar, that speckling on the bottom. Depending on your wort, there might be some extra trub on the bottom. If it was a bacterial infection....you wouldn't be able to really see it. If it was a mold infection... it'll be most likely visible on the top. I wouldn't worry about...

I work in a lab too where we use cryovials. It's best to make sure that if you're using glycerol ( be sure it's sterile also!) that it's at least 15% final volume to prevent any cellular membrane damage. As everyone has said, be sure to do a slow freeze and then have it placed in -80C or liquid...