I am culturing up the dregs of a bottle of Orval and doing counts to check progress. I can see what appears to be two distinct yeast populations. A large "saccharomyces looking" population, and a smaller population. This would make sense since there should be primary fermentation yeast in there as well as 3 Brett strains?
I am counting them separately since I want to see the Brett multiply (assuming the small ones are Brett).
I started with 250ml total volume (with the dregs) of approx 1.025 wort on a stir plate.
2 days later I dumped that into a total volume of 1,000ml 1.040 wort and added constant air with a stone (I do this 4X step up with ale yeast and get a 10X increase in cell volume usually)
I got a 2X increase with the 250ml propagation.
Can anybody weigh in on this regiment? I assume Brett likes O2 for sterol synthesis just like Sacc?