I finished up brew day yesterday, and already have some activity in my first batch. My question is I made a second starter for my second rubbings but I don't think I started it early enough because the krausen was just starting to form so I rehydrated some US 05 and pitched it. Can I just wash my yeast from second starter?
YOU shouldn't need to wash the yeast from a starter, no trub and little proteins, little to no autolysis assumed. If I am understanding your question. I would decant the beer off and put the small quantity of residual yeast in a fresh starter of adequate size and step it up a couple times, then crash in the fridge for your next brew and starters. You can save it in the fridge short term or read up on freezing yeast with glycerin. I always propagate an extra starter when i step mine up. 2-2L starters for pitching, 1-1L for growth/retain and splitting into 3 the next brew cycle. this way I don't ever wash yeast and always have a fresh low stressed start handy.