Just my curiousity for the day... The general rule seems to be not to use re-use yeast more than 4 times. So, how do wyeast and the other guys 'make' yeast? I would imagine they just keep breeding it from the yeast they already have, but there's no way you could sustain that without going way past 4 generations... Unless they do something like cryo freezer first generation yeast in the millions and then breed more batches from one cell at a time...
Anyone know?
Anyone know?