So yesterday I boiled up 12 gallons of sweet wort and used the 3 week old yeast slurry that I harvested the day before. Chilled the wort down to 68 F and split the batch into 2 buckets. Then I pitched half of the slurry in one bucket and the half in the other bucket. Then into the fermentation chamber. So the theory is that this is the exact same wort and the exact same yeast slurry so the 2 buckets should act the exact same way. Well, one bucket took off in about 3 hours, bubbling away. The other one nothing yet after about 24 hours. I know, bubbling is not a sign of fermentation but it sure makes you feel better when you see it. We will see what happens in a day or two I guess. This hobby always has surprises.