When harvesting out of bottles, start with a very small amount of wort (ex. 200mL for 5 gal), when you see activity (24-48 hrs), then pitch into 1800mL to make a 2000mL starter, 24 hours later you should be ready to pitch into 20000mL or 20L (approx 5 gal.). As long as you wiped the bottle mouth...