Search results

Here are some long overdue photos. These are all wild yeast (and other stuff) that were captured by rubbing different things on the plates. Juniper berries, pine cones, Russian sage flowers, rotting crab apples, etc. All plates have cycloheximinde (10-20 ug/ml) and the blue looking plates...

I'm not sure how this relates to others experiences using wort to harvest yeast and bacteria, but I collected wild yeast using agar plates. I did everything from leaving the plates outside and uncovered overnight, to covering them with cheesecloth, to simply rubbing fruit, flowers, and other...

The photos are at 64X. They should be pure since the grows were from single colonies after a few rounds of selection. I'll take a few photos of the plates, but surprisingly, colony morphology doesn't seem all that different between the different isolates I have. That said, I'm not a yeast...

Got 2 new isolates that smell super funky when grown in WLN media. I'm going to do a few mini batches this weekend with 3 different isolates and see what flavors/aromas these guys produce with barley! I'll update in a few weeks. Such a different morphology between these two isolates. Photos...

Another question. I have over 10 different pure Brett cultures (or at least I think so) isolated at various times and places over many hundreds of miles. While I bet most are the same, I thought I might get a few different species or even sub types based on the different time and location of...

dinnerstick- Not sure based on morphology, but I found that plates with chloramphenicol (25ug/ml) were really helpful in getting rid of bacterial contamination and cycloheximinde (10-20 ug/ml) were useful for enriching for potential Brett. I've started making plates with both when I try to...

jaapie- Thanks for the link to your post. I isolated 10 more cycloheximide resistant clones in the mountains this weekend. Time to start seeing what kind (hopefully different) flavors they produce. Should be interesting.

Yeah, there are some simple PCR assays that will distinguish Brett from others. As posted above and this paper as well - http://www.ncbi.nlm.nih.gov/pubmed?term=Tessonni%C3%A8re%20h Also, this lab will do identification. Not sure of costs. http://www.etslabs.com/ The cyc resistant clone in...

This thread is awesome. I have an experiment on going and will post photos soon, but I had a question. I have a few strains of wild yeast that metabolize bromocresol green - so they are white/opaque when grown on BCG, yet they are sensitive to cyclohexamide at 10-20 mg/L. From what I've...

Thanks for the replies. I was essentially trying to keep potential contamination down by using selective media. I just want to store and reuse a few strains I use a lot. daksin - Thanks. That's interesting that they don't use any sort of selection.

Does anyone know what selective media Wyeast Labs uses to grow their strains? For example, maybe the American Ale 1056 strain is URA-. I'm sure not all are the same and it's a fairly easy experiment to do, but I'd figure ask here to see if anyone knows or has done this before I invest the time...

i want one

Thanks. I just found that thread!

Just a follow up to jalgayer's questions. How much fruit, maltodextrin, DME or honey would you add at a time? If adding maltodextrin or DME do you dissolve it in water first? Kind of an unrelated question, but the current lambic I have going is for my wife who likes the sweetness of...