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I just saw this at Williams Brewing - new to me: https://www.williamsbrewing.com/Home-Brewing-Equipment/Kegging-Equipment/Tubing-Tubing-Accessories/Copy-of-316-Clearflo-Ag-47-Antimicrobial-Beverage-Tubing 3/8" Clearflo® Ag-47 Antimicrobial Transfer Tubing Are you looking for better quality...

Maybe I'm too conservative, but 3 volumes is generally considered the limit for pressure in standard beer bottles. I go with 2.8 volumes to be on the safe side, even though I'm probably not getting the maximum quality in the hefe.

I think I use S-189 on half of my lagers - really like it.

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Great start. Welcome.

Good job getting your old account back. Welcome back.

Welcome from NC.

I agree 100% - continuous improvement.

You can see from the posts above that there are quite a few items involved here. There are a lot more, too. I highly recommend getting How to Brew, E4. As it suggests, ideally you would read the first ten chapters before brewing. But at a minimum read the first chapter before brewing. I'll note...

Efficiency was low, and top-off water volume was low. To some extent, they cancelled in terms of extract concentration.

IMO it's best to just take two gravity samples, 3 days apart, rather than sampling three days in a row. Less chance for contamination and oxidation.

I agree - not a proven fact. But several posters on this thread indicate it can happen. I’m wondering if some suspended solids (e.g. cold break) in the wort are setting and causing the stratification effect. Your sample looking stratified might be an indication of this. That might even indicate...

I’m really surprised that a true solution, as opposed to a suspension of solids, would stratify noticeably in the few minutes before taking a sample. Now I’ve got something else to worry about. (Not really a believer in Papazian’s DWHAHB.)

Welcome.

Besides changes in equipment, there has been a lot of progress in ingredients and process. You will benefit greatly from reading as much as you can find. Palmer's How to Brew, 4th Edition is the best that I'm aware of. It's from 2017, so not completely up-to-date, but it will help supplement...

Once you're satisfied with your tap water chemistry, you might be interested in AJ DeLange's A Brewing Water Chemistry Primer: https://www.homebrewtalk.com/threads/a-brewing-water-chemistry-primer.198460/

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This is what I use: https://www.ritebrew.com/product-p/863136.htm Edit: I use it for bottling - not transferring wort to the fermenter.

Welcome to HBT.