What's in your yeast farm?

Homebrew Talk - Beer, Wine, Mead, & Cider Brewing Discussion Forum

Help Support Homebrew Talk - Beer, Wine, Mead, & Cider Brewing Discussion Forum:

This site may earn a commission from merchant affiliate links, including eBay, Amazon, and others.

ArcaneXor

Well-Known Member
Joined
Nov 10, 2007
Messages
4,502
Reaction score
128
I am finally starting to build up a bank of harvested and washed yeast for reculturing and blending, and was curious what others on these boards keep on hand for their house yeasts.

I have got (or am planning to store in the near future):

WY 2565 Koelsch
WLP 530 Abbey Ale
US-05 American/Cal Ale
S-04 Whitbread
WY 2124 Bohemian Lager

and thinking of starting to look into the various bottling strains and culturing some of them up.
 
I keep masters in distilled water, and supply stocks on slants.

I so far have:

wlp 001 california ale
wlp 029 german ale/kolsch
wlp 830 german lager
wy 1332 northwest ale
wy 3655 belgian schelde
wy 2112 california lager

waiting for plate space:

wlp 565 saison ale
wy 2042 danish lager
wlp 072 french ale

Also got lots of sterile plastic tubes for trading if anyone is up for it.
 
Already harvested:

Wyeast 1028
Wyeast 1056
Wyeast 1968
White Labs WLP023 Burton Ale

On the way in an order and will be harvested as well:

Wyeast 3068
Wyeast 1318
Wyeast 1214
White Labs WLP022 Essex Ale
White Labs WLP051 California V

And that will hold me off until at least November.
 
and thinking of starting to look into the various bottling strains and culturing some of them up.

BTW, I've noticed with Wyeast 1028 in a Dry Stout that I made that it's a really sticky yeast. When I pour that beer, the yeast sticks to the bottom and I get to pour the entire beer without having to leave dregs in the bottle. I don't have extensive experience with this yeast but it might be worth a look if you like British styles and want a good bottling yeast too. It gets a high level of attenuation but is still sticky, at least in this beer.
 
Here's what I have:

White Labs
WLP001 - California ale
WLP002 - English ale
WLP004 - Irish ale
WLP007 - Dry English ale
WLP011 - European Ale
WLP037PL - Yorkshire Square ale
WLP380 - Hefeweizen IV ale
WLP400 - Belgian Wit
WLP410PL - Belgian Wit 2
WLP500 - Trappist ale
WLP515PL - Antwerp ale
WLP530 - Abbey ale
WLP565 - Belgian Saison
WLP775 - English Cider
WLP833 – German Bock Lager
WLP840 - American Lager
WLP862 - Cry Havoc

Wyeast
WY1272 - American ale 2
WY1318 - London Ale 3
WY1450 - Denny’s Favorite 50
WY1762 - Belgian Abbey II
WY1764PC - Rogue Pacman Yeast
WY2124 - Bohemian Lager
WY2206 - Bavarian Lager
WY2247PC - European Lager
WY3522 - Belgian Ardennes
WY3739PC - Flanders Golden ale
WY4184 - Sweet Mead

BrewTek
CL-160 - British Draft Ale
CL210 - Scottish Bitter
CL300 - Belgian Ale #1
CL320 - Belgian Ale #2
CL340 - Belgian Ale #3
CL380 - Saison
CL400 - Old German Ale
CL450 - Kolsch
CL900 - Belgian Wheat
CL920 - German Wheat
CL930 - German Weiss

Cultured from bottled beer
Russian River Temptation
Boulevard Saison-Brett

Most are from my own banking from tubes/smack packs, and a few are from trades. I'm always open to trading if you have anything not on my list or if you just want to expand your bank.

Cheers,
Mike
 
Notty, Montrachet, S-23 for dry yeasts I haven't used yet.

Stuff I have cultured:
4x WLP001 Cali Ale
2x Wyeast 2565 Kolsch Ale
2x Wyeast 1028 London Ale

I used two jars each of my last two batches of Kolsch & London Ale. The Kolsch I doubled up because I planned to ferment around 57°F, and the London Ale I doubled up because it's my house porter, which has an OG of 1.072. For whatever reason I didn't culture anymore of the Kolsch, but once my porter finishes I plan to snag another 4 mason jars of it.

I think my mix covers me for anything. I have to Kolsch to pseudo-lager, wheat beers, or fruit beers. The Cali-ale for anything American, and the London Ale for British Ales.
 
I have a Cynmar order arriving this week.

So far I have made starters oversized and kept a 1/2 pint of

WLP001 California Ale
WLP005 British Ale

I plan on harvesting and propogating.

I also have WLP500 Trappist Ale and will order WLP 850 Copenhagen Lager. I am looking at a variety of yeasts with the broadest applications to cover all my brewing needs. I am still looking at slanting versus freezing as my ranching technique.
 
BB, how do you brew enough to keep that many cultures active?

I keep them all on slants in 2ml microfuge tubes so it is really easy to reslant them every 4 months. I also keep glycerol stocks in the -80C freezer at work, so I may let the slants go except for the ones that I use often if my club doesn't start using them.

@krops13 - whatcha got?
 
I've got 2565 (kolsch), 3068 (weihestephanen),and 3711 (french saison) in glycerin in the freezer.

I've got beers on Pacman, and us-05 right now, which will both get harvested.
 
I have:
Cali Ale I and V
Irish Ale
Hefe

From bottles:
Bell's
Pacman

I keep a slurry in a pint bottle in the fridge, so trades maybe needs to be a local swap.
 
Bell's house ale
Bell's White
Schlafly Wheat
Wyeast 1028 London Ale (Worthington White Shield)
Wyeast 1056 American Ale (Sierra Nevada)
Wyeast 1084 Irish Ale (Guinness)
Wyeast 1272 American Ale II (Anchor Liberty)
Wyeast 2035 American Lager (August Schell)
Wyeast 2206 Bavarian Lager (Weihenstephan 206)
Wyeast 3787 Trappist High Gravity (Westmalle)
Wyeast 3864 Canadian/Belgian Ale (Unibroue)
Wyeast 3944 Belgian Witbier (Celis White)
 
Hope this thread gets active again! I love yeast ranching although its starting to get time consuming...

In distilled water and slants:

WLP 830 German Lager - Weihenstephan 34/70
WLP 029 German Ale/Kolsch - PJ Früh, Köln
WLP 001 California Ale - Sierra Nevada
Wyeast 1332 Northwest Ale - Hales Brewery in Seattle via Gales Brewery UK
Wyeast 3655 Belgian Schelde - ?
Wyeast 2112 California Lager - Anchor
Wyeast 1728 Scottish Ale - McEwans
WLP 565 Belgian Saison - Brasserie Dupont (Saison Dupont)
WLP 004 Irish Stout - Guinness
Wyeast 2000 Budvar - Budvar
Wyeast 1010 American Wheat - Widmer vis Zum Uerige
Wyeast 1968 London ESB - Fullers
Wyeast 2206 Bavarian Lager - Weihenstephan 206
Bell's house ale yeast harvested from bottle of Oberon
WLP 380 Hefeweizen IV - Weihenstephan 66?
Wyeast 3711 French Saison - Brasserie Thiriez
WLP 530 Abbey Ale - Westmalle
WLP 007 Dry English Ale - Whitbred-Dry
WY 3739 Flander's Golden Ale - Ertwelde, Gulden Draak?
WY 2007 Pilsner Lager - Budweiser
WY 2042 Danish Lager - Miller via Carlsberg
Wyeast 2247PC European Lager
WLP653 Brettanomyces lambicus
Wyeast 2308 Munich Lager - Wisenschaftliche Station #308 (Munich)
WLP802 Czech Budejovice - Samsons via Budejovicky Mestansky Pivovar
WLP002 English Ale - Fullers
WLP500 Trappist - Chimay
WLP 510 Belgian Bastogne - Orval
 
I have just started my frozen bank. All I have thus far is;
WhiteLabs 029 German Ale/ Kolsch
Wyeast 2252PC Rasenmaher Lagerbier
Wyeast 1099 Whitbread

*all 1st generation in frozen 16ml vials.
 
I have 1338 stocked up. I'm currently brewing with WLP575 so I'll have that on hand.

I need to culture a saison strain for a future saison later this summer. I just purchased some small vials to create a frozen bank so I can start building up a supply.
 
I actually just started yeast washing about a month ago. I have Wyeast 1056 - American Ale, and I will be washing Wyeast 1098 - British Ale tonight.
 
WLP 001 Cali Ale
Wyeast PacMan
Danstar Nottingham
Fermentis T-58

I'm looking to expand like crazy when I get around to culturing my stocks!
 
I'm just getting into the yeast bank thing and I'm hoping that someone will take pity on me and help me out by inoculating slants for me. It seems to only makes sense financially if you've got several strains in your bank, but I'd be willing to ship you a batch of sterile, prepared slants with a pre-paid return box/envelope if you'd inoculate them for me. My bank is pretty weak at the moment, so I'm looking for any ale yeasts you've got.

My bank:
Wyeast 1272 - American Ale II
Wyeast 1338 - European Ale

Anything you've got would be appreciated. Once I've got a bank up and running I'll return the favor to anyone that asks.

PM me if you can help out.

Aaron
 
I just collected my first strain, wlp830. I also will be collecting wlp001. I'm keeping them in my lager freezer. How long can I keep them at 35F?
 
From the incredibly helpful Maltose Falcons article by MB Rains (http://www.maltosefalcons.com/tech/yeast-propagation-and-maintenance-principles-and-practices):

Table 6. Summary of methods for yeast storage.

Method Shelf-life (years) Advantages /Disadvantages

Liquid media 0.5 Convenient but low viability and stability, questionable purity

Agar plate 0.2-1 Pure cultures but unreliable shelf-lives.

Agar slant 1-2 Easy, reliable, but moderate shelf-life

Agar stab 2-4 Easy, reliable, good shelf-life, but messy.

Dried 3-6 Inconvenient, requires purification.

Frozen >5 Need special freezer or liquid nitrogen

In other words, if you're refrigerating your slants at 35F you should be good for 1-2 years. Check out that article. It has everything you need for yeast propagation and storage. Hope that helps.
 
That's a very useful article (and one I have read often before) however you can't just include the table since it's inaccurate without reading the article:

Plates/slants are essentially the same thing, the surface area, if it is airtight and how it is stored are really the only differences, so the storage time is really the same.

Slopes are useful, however after even a few month(s) you will get yeast dying and after a year the article suggests reculture. However some people have kept slants for up to 4 years.

Even the author says that stabs are not used for yeast and his life-estimate is a guess, and they're not really messy just difficult to work with. You can extend the life of slants by using using mineral oil to cover the surface, however that is messy.

The author suggests that the shelf life of dry yeast has not been determined, however the process invites contamination, and also yeast mutations.

The only 'special' thing you need to freeze yeast is a frost free freezer, which can easily be accommodated for by using a styrofoam or similar container to store your yeast in (to protect it from the freeze/thaw cycles).

In terms of long-term storage of yeast, the more recent TBN podcast on the topic is probably a little more up-to-date and useful.
It's not just storing the yeast that is important, but also keeping it free from mutation and changes, some methods do that much better than others. Also since we are able to (essentially) reculture from very very small numbers of viable cells many storage methods can be used if long-term-storage is the only criteria and if the yeast is recultured carefully.
 
I have just started but...

WLP001- California Ale
WLP568- Belgian Saison Trappist Blend
Wyeast 3638- Bavarian Wheat
Wyeast 1335- British Ale II

Ommegang Proprietary Strain

Adding to the list-
Wyeast 3538PC- Leuven Pale Ale, Private Collection
WLP001- London Ale

Also, I will just put in I use the Glycerol/Yeast Nutrient freeze down approach at -20C approach. Less space and longer shelf life.
 
wow - some of you have lots of yeast!

I keep only a few

WLP 001 California Ale
WLP 810 San Fran
WLP 833 German Bock

WY 1450 Denny's Fav 50
WY 1099 Whitbread
WY 1056 American Ale
WY 1007 German Ale
 
My experiments with frozen yeast were largely unsuccessful, probably because of temperature fluctuations in my crappy freezer. My washed/fridge-stored yeast has been very reliable - it may take a day or so to get going on the stirplate, but once the stepped-up starter is pitched, it's as good or better as new. I always harvest directly from the yeast starter before pitching into the main wort to minimize yeast stress.
 
Wolfy,

Good points. All methods are viable and have advantages/disadvantages. I don't suggest that one is better than another, although, I believe that frozen (in the correct manner) would probably last the longest. My purpose with the post was simply to provide some information regarding suggested shelf lives of different methods as an answer to the question posed by hollywoodbrew. In a world of conflicting information regarding yeast propogation, the Maltose Falcon article is, IMO, one of the most reliable.

Do you have a link to that TBN podcast on yeast? I'd love to listen to that. Any other info on this topic that you can point me to that you feel is worth while?
 
Just re-starting...
Wyeast 3068 being done tonight.
Wyeast 2575PC Kolsch II next week
Wyeast 1056 harvesting this weekend.

Harvested Notty & S-05 ? wow.
 
Good points. All methods are viable and have advantages/disadvantages. I don't suggest that one is better than another, although, I believe that frozen (in the correct manner) would probably last the longest. My purpose with the post was simply to provide some information regarding suggested shelf lives of different methods as an answer to the question posed by hollywoodbrew. In a world of conflicting information regarding yeast propogation, the Maltose Falcon article is, IMO, one of the most reliable.

Do you have a link to that TBN podcast on yeast? I'd love to listen to that. Any other info on this topic that you can point me to that you feel is worth while?
The article is great, however since we can reculture from (virtually) one viable cell, many storage methods are much the same in terms of being viable after years. However, preventing yeast mutation (which is not really mentioned in that article) is possibly more important than the viability after long term storage. I have a non-frost-free-freezer, so have just started freezing samples, hopefully to keep them more pristine, and also because slants get tedious the more yeast you get - maybe I'll update this in 5 years when I know more about the viability/mutation over time. ;)

The Brewing Network - Brew Strong - Yeast Library 3-8-10
(is what my MP3 says, it's 5.30am here, you can find the link) :)
 
Just re-starting...
Wyeast 3068 being done tonight.
Wyeast 2575PC Kolsch II next week
Wyeast 1056 harvesting this weekend.

Harvested Notty & S-05 ? wow.

I am also planning an adding the Koelsch II to my library. Harvesting Notty makes sense since they have had some quality control issues in the recent past (I still have several satchels with holes in them lying around) - otherwise, it's too cheap to save money when figuring in the DME cost for the starter. I switched from harvested US-05 to 001 for my American Ale strain, since the liquid yeast is supposedly slightly cleaner.
 
Back
Top