Anyone in Central MA with a small functional lab?

I'm looking to find if someone does what White Labs does on a very small rudimentary scale to bank yeasts. Not so much to store them but more to identify, grow, isolate, etc. If per chance a nice wild yeast was found I would love to keep it for longer periods of time. Just an inquiry is all

Storing in the back of a freezer with 10% glycerin works well for me. I wouldn't think you would have to do cryogenic freezing unless you are considering storing the yeast for decades.

As for identification, there are a couple PCR tests for finger printing yeasts. I could send you a couple of papers if you are interested.

How long have you done storage in the freezer with good results? Amount of yeast to store an issue?

I'm not too interested in the storage aspect as I am in the isolating the good from the bad. If a wild sacc/Brett/ was mixed with an aggressive companion I would want to preclude it. I guess I should stick to my simplistic ways as that makes me more happy. Just pitch and move on from generation to generation.

WoodlandBrew said:

As for identification, there are a couple PCR tests for finger printing yeasts. I could send you a couple of papers if you are interested.

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And I can sell you a broken thermocycler.

WoodlandBrew said:

As for identification, there are a couple PCR tests for finger printing yeasts. I could send you a couple of papers if you are interested.

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Is that Polymerase Chain Reaction?

SnakeRidge said:

And I can sell you a broken thermocycler.

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Pffffft!

djbradle said:

Is that Polymerase Chain Reaction?

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Yep. Read up on the guy who invented and won the Nobel Prize for it, Kary Mullis. Interesting dude.

Might do that with the next free moment of time, thnx

Woodlandbrew, if you don't mind sending pdf's on 'em that'd be great. I'll pm my email.

There are some good free papers I need find the URLs and I'll post them.

SnakeRidge said:

And I can sell you a broken thermocycler.

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I'd be interested in a used one if it worked.

WoodlandBrew said:

There are some good free papers here are a couple:

http://onlinelibrary.wiley.com/doi/10.1002/jobm.3620330609/abstract



http://www.ajevonline.org/content/45/1/86.short

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Thanks!

I'll stick to brewing with cerevisiae and not those .....anus ones. Lol!

djbradle said:

Thanks!

I'll stick to brewing with cerevisiae and not those .....anus ones. Lol!

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I need to double check those links. I don't want to waste your time. I should be able to dig up the good ones tomorrow.

Taken from the first link I jest....
".....allowed us to discriminate 23 biotechnologically important strains of the yeast Saccaromyces cerevisiae and to distinguish them from strains of S. pastorianus, S. bayanus and S. willianus."

djbradle said:

Taken from the first link I jest....
".....allowed us to discriminate 23 biotechnologically important strains of the yeast Saccaromyces cerevisiae and to distinguish them from strains of S. pastorianus, S. bayanus and S. willianus."

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Here is a good paper on PCR:
http://femsle.oxfordjournals.org/content/221/2/249.abstract

And Siebel offers PCR testing for about $100 when I asked. You'll have to contact them for details, but here are the services:
https://www.siebelinstitute.com/services/yeastmaintenance/

Can your HERMS double as a thermocycler?