Cell calculation

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DurtyChemist

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Washed some yeast and stored it in 50 mL centrifuge tubes. I have around 5 mL in each tube and my last calculation says it was only 65% attenuation. (1.047-1.016)/0.47

1. I'm wondering how I can calculate how many cells I will have if I use 5 mL of my stored yeast for a 1 L starter for an ale

2. I'm wondering if the yeast is junk, I didn't aerate enough or if I can possibly bring up the apparent attenuation closer to the 73% area.
 
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