Yes, I take it out of the fridge, pour off most the beer that the starter produced, I save only enough to swirl the yeast back into suspension for pitching, but I let it come to room temp prior to yet swirl, and pitching. IMO it's not really advisable to pour the whole starter in because the beer in your starter is aerated, and not of the best caliber. There is still yeast in the beer you created with the starter, but most has formed a yeast cake on the bottom of the flask, and is more than enough to get a 5 gal batch kickin. GL, and happy brewing, making yeast starter is easy and fun, just keep sanitary, it's like brewing a mini batch of beer.