Thanks for the insight. It's a rough clone (all grain) of arrogant bastard. The OG wasn't really specified so I presume it would be around 1070 or so.
I could do a second yeast starter after this is done. I guess, I was more concerned with whether the yeast would actually do they are supposed to do in a yeast starter. If that made sense.
Thanks again. I'll read those links you posted.
Your yeast will do what it is supposed to. Even in a starter that small. It just wont do as good a job as it should.
With a Gravity of 1.070 I'd say you should in fact step up your starter and make a larger one.
How have you aerated your starters thus far?
Since you've said that this is only your 2nd one I'm going to assume you have a basic set up and have just been shaking your starter intermittently to allow your yeast to get some of the oxygen they need to reproduce. If you don't have a stir plate and haven't even been shaking your starters, I recommend starting to do that whenever you have time. I would leave my starter close to my beer fridge so whenever I was home and I would grab a beer or walk past it, I would give my starter a 5 second shake.
With a gravity of 1.070 and your first starter's volume at about .5L right now, your next "step" should be a volume of 1.30-1.40 liters. To achieve a gravity of 1.037 in this volume you should add 7.1 oz of DME to your boil.
To properly step up your starter, you should put your .5 liter starter in the fridge the night before you plan on "stepping up". The day of you should get your water to a boil, as you are boiling you should be decanting or pouring out the used wort out of your starter. Being sure to leave all of your yeast at the bottom of your container. After you have decanted the old wort, allow your new fresh wort to cool. After its cooled to room or "ale" temp, just pour it on top of your original yeast cake. Let it do its thing for about 24-36 hours (being sure to shake it as often as possible during this time) and you will have all the yeast you need to properly pitch and ferment your clone.
If your current container isn't large enough to hold about 1.5-2 liters of liquid comfortably, you should be sure to sanitize one that is large enough and be sure to leave enough wort in your original container to mix your yeast cake back into suspension. Once you stir all the yeast up into your small amount of old wort, pour all of the contents into your newer larger vessel and continue with the steps above.
Once you get further into making starters and acquire or build your own stir plate, you will be able to get more yeast out of much smaller starters. Saving you time and money that you would be using to buy the extra DME.
Check it out if you have any interest.
https://www.homebrewtalk.com/f51/my-stirplate-cheap-easy-build-86252/
cheers.
Good luck. Hope this little step by step break down helps.
Cheers.