I store my stocks in 2ml cryovials at -80c. When I want to grow stocks, I scrape a pipette tip worth of the solid into 3mls of 10% dry malt extract (100g/L) and shake at 30c/200rpm for 24-36h, basically until turbid. Then I inoculate 50mls of 10% DME at 18c/200rpm for 24h and then inoculate 1...