wegz15
Well-Known Member
So I started stepping up a yeast slant. I put it in a 50ml starter for 24 hours. I then stepped it up to 1l starter for 48 hours.
I did a hemocytometer count today with a 1:10 dilution. In 5 boxes I counted 204 cells. According to my math this would be roughly 4 billion cells(408x10^4 cells/ml x 950ml=~4b cells) in the 1L starter (~950ml), correct?
My OG is calculated at 1.083. For this I calculated I need roughly 300 billion cells.
I just started stepping up my 1L starter to a 2L starter taking 20ml from the 1L and added it to the 2L. I plan to leave this for about 36 hours and do another cell count.
Am I doing the right math and step?
I did a hemocytometer count today with a 1:10 dilution. In 5 boxes I counted 204 cells. According to my math this would be roughly 4 billion cells(408x10^4 cells/ml x 950ml=~4b cells) in the 1L starter (~950ml), correct?
My OG is calculated at 1.083. For this I calculated I need roughly 300 billion cells.
I just started stepping up my 1L starter to a 2L starter taking 20ml from the 1L and added it to the 2L. I plan to leave this for about 36 hours and do another cell count.
Am I doing the right math and step?