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Old 01-18-2011, 02:15 PM   #401
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Quote:
Originally Posted by ekjohns View Post
most micro centrifuge tubes come in bags of 500. $60 seems way over priced. Buy them from www.fishersci.com for a much cheaper price
same price; just no qty listed on amazon, looks to be 200/box

NC9521468

I think these are the same ones...

EDIT: Fixed link
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Old 01-18-2011, 02:24 PM   #402
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Link didn't work: try posting catalog number

-matt

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Old 01-18-2011, 02:45 PM   #403
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those are for RNA and DNA and pre-sterilized which makes them much more expensive and not needed for this application. If you have a pressure cooker just sterlize them yourself and order 02-681-258 from fisher (Pack of 500 for $23.83)

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Old 01-18-2011, 02:47 PM   #404
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as a side not if you would not like to sterlize them yourself you can use these 02-681-5 they are sterile and individualy wrapped so you can use them as needed without special handeling. Pack of 250 for $18.13

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Old 01-18-2011, 02:57 PM   #405
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those are for RNA and DNA and pre-sterilized which makes them much more expensive and not needed for this application. If you have a pressure cooker just sterlize them yourself and order 02-681-258 from fisher (Pack of 500 for $23.83)
THANK YOU! This is why i posted rather than just ordering...so these won't melt in the pressure cooker? I did a little reading and found the PET ones are not autoclavable. But these are polypro. Any issue with the snap caps instead of screw on caps? don't want them to snap open and spill...

500 for $23 is much better than $100 for 240 of the glass ones!
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Old 01-18-2011, 03:54 PM   #406
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the snap caps do not open for common use. every science lab uses these tubes for various applications. When freezing in -80 your suppose to keep cells in screw cap Cryogenic Vials which are much more expensive. However, with these tubes if you store them at -20 C they will work perfect. If you store them at -80 C let them warm up slowly and you will be fine. Screw caps are designed to be used for fast thaw and freeze such as submersion in N2.

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Old 01-18-2011, 08:36 PM   #407
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Thanks ekjohns, very much appreciated!

So i have another question..more about starters than anything else...
Looking at mr malty calculator it says I need 323 billion yeast cells & 5.23L for my starter for the beer i'm brewing this weekend (1.090 OG Belgian Noel from AHS)

the other beer we're brewing this weekend a DFH60min says i need 219billion cells & a 1L starter. How do i know i have enough cells?

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Old 01-18-2011, 11:38 PM   #408
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if your are buying yeast from wyeast or white labs there is a date on each. wyeasts date is the date it was packaged, white labs date is 3 months after it was packaged (if the date is march 1st it was packaged Jan 1st). Use those dates to calculate how big of a starter you need. Dont worry about the actual number of cells

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Old 01-20-2011, 05:42 PM   #409
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Newbie here.....haven't read through the 40 plus pages here

Would it be possible to store the yeast in used 12oz soft drink bottles? Would dunking them in starsan be enough sanitation?

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Old 01-20-2011, 10:20 PM   #410
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Newbie here.....haven't read through the 40 plus pages here

Would it be possible to store the yeast in used 12oz soft drink bottles? Would dunking them in starsan be enough sanitation?
Nope. Because we are working with small amounts of yeast everything need to be sterlized to prevent bac taking hold. If your wanting large volumes use 12oz canning jars which can be sterilized
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