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Thanks for the guidance on starter volumes - the 10x progression seems pretty reasonable. I might try some cell counts to get a ballpark number for cells in a slant — microscope is on order. Any recommendations on how to approach this? I would probably start some new slants, trying to cover as...

I’m a homebrew noob learning to be a yeast rancher. I followed the instructions on inoculating slants from the sticky thread in this forum - so far so good. It looks like the usual way to create a starter from said slants is to use the entire slant. First question: what is a good assumption for...