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  1. S

    Interesting genome sequencing of some yeasts

    I've downloaded all the data for all the WLP, Wyeast, Fermentis and Lallemand strains and have them running through my pipeline. Creating a list of Saaz and Frohberg lager strains is on my list of things to do (should be ready tomorrow). An updated family tree should be ready during the weekend...
  2. S

    Interesting genome sequencing of some yeasts

    Thanks sykesey for sharing! Very interesting stuff! The organism/hybrid designations are based on whole-genome sequence data and should be accurate. This is from one of the top yeast genomics labs in the world, so they know what they are doing. Vale71, this is a completely separate study to the...
  3. S

    White Labs Yeast Vault

    I've gotten over 90% attenuation all four times I've used WLP026. Gravity from 1.060 down to around 1.004. Fermentation temperature was twice at 20C, twice at 12C. WLP026 is diastatic (has the STA1 gene), and it has the full STA1 promoter sequence (so the STA1 gene is expressed at 'normal' levels).
  4. S

    Isolated Yeast (Tree House): How to Identify and Characterize?

    I'm wondering if the strain similar to WB-06 is actually WLP644? The fingerprint Chris (famouslastworts) got is almost identical to one I got of WLP644 back in the days when I was confirming my Conan × WLP644 hybrids (http://beer.suregork.com/?p=3747). We also know through genome sequencing that...
  5. S

    anticipating having to restart a stuck WY3724 saison

    This is a bit off-topic, but this here is a prime example of how our findings in this recent preprint ( https://www.biorxiv.org/content/10.1101/654681v1 ) can explain some of the scenarios homebrewers encounter with saison strains (I started a separate thread a couple of weeks ago with the main...
  6. S

    Solving the mysteries of diastatic brewing yeast

    Thanks! I believe the standard is to use end-point PCR, but I believe the more sophisticated systems use qPCR for improved detection limits.
  7. S

    Solving the mysteries of diastatic brewing yeast

    So this is not super relevant to homebrewers, but I thought it might at least interest some of you. This is related to diastatic brewing yeast (i.e. the yeast formerly known as Saccharomyces diastaticus). About a year ago I set out to elucidate the reasons behind why some strains carrying the...
  8. S

    Conan x WLP644 hybrid

    I have the hybrids safely stored in our -80 freezer, so anyone interested in trying these can send me an email (my username here at HBT at gmail.com).
  9. S

    From the lab - family tree of White Labs yeast

    Yes the latest updated diagram only contains the WLP codes for the confirmed strains. Prices for whole genome sequencing are going down all the time. At the moment, you can sequence a strain for as low as 100 USD (Illumina sequencing, ~30x coverage).
  10. S

    WLP051 possible failure

    Phil just pointed out that White Labs have released a new 2018 catalogue, and in it they reveal WLP051 is actually S. pastorianus! Very interesting, but not very surprising (with lager yeasts revealed as ale yeasts). Will have to check if it is Saaz or Frohberg. Sorry for going off topic!
  11. S

    From the lab - 1002 genomes

    Yes, looks like DBCPG6694 and 95 may mistakenly have swapped places in the 1002 genomes paper. Regarding WLP530, I'm really not sure what is going on. I'll keep looking at the data though and see what I find out. I'll get back to your email later today or during the weekend!
  12. S

    From the lab - 1002 genomes

    Here are some preliminary results. If the closest match is the clade rather than a single strain from the Gallone strains, then there was no very close match. Hopefully the formatting looks ok :) Some results are quite strange, e.g. WLP530 being closest to Beer095 (I wonder if it really was...
  13. S

    Zymurgy March/April 2018 - The Origin of Ale Yeast Species (DNA Testing)

    Yes, I noticed WLP099's inability to use maltotriose already a couple of years ago and wrote about it here: http://beer.suregork.com/?p=3850 It definitely looks like WLP099 is a blend, since people have been reporting very high attenuations even in all-malt wort (impossible without maltotriose...
  14. S

    Zymurgy March/April 2018 - The Origin of Ale Yeast Species (DNA Testing)

    As Mer-man mentioned, together with help from others, I've tried to decode most of the White Labs strains from that study (based on genome data published elsewhere): http://beer.suregork.com/?p=3919 The blog post also contains a spreadsheet with brewing-relevant phenotypic data of the...
  15. S

    Isolated Yeast (Tree House): How to Identify and Characterize?

    One group of strains that seem to be genetically quite close to the German wheat beer yeasts, meaning they would yield similar fingerprints, are the lager yeasts (see here). Looking closer at the fingerprints of S-23 and W-34/70 (lanes 14 and 16), it looks like they could be a match for the...
  16. S

    Pressure-canned starter wort; why so dark?

    My autoclaved wort (made from extra light DME; 100 g / L) always ends up looking the same (lab autoclave, 121C for 15 minutes, temperature probe in liquid of equal volume; whole run time depends on liquid volume, but usually 45 minutes to 2 hours). As ESBrewer mentioned, the higher temperatures...
  17. S

    From the lab - family tree of White Labs yeast

    Thanks for posting a thread about the topic :) it sure is an interesting topic, and all the phenotype data should hopefully be very valuable to the (home)brewer now that the strains are known (at least with some confidence). Special thanks to all the contributors in the comment section in my...
  18. S

    Ale yeast for Lager recipe?

    While this thread seems to be concentrating on 'kveik' yeasts at the moment, I thought I'd go back to the original question (ale yeast for lagers?) and bring up some interesting information about one of the commonly used homebrew lager strains: WLP800. WLP800 is supposedly a lager yeast, and...
  19. S

    Isolated Yeast (Tree House): How to Identify and Characterize?

    Cool, didn't realize you were in the latest send-out :) What primer pair did you use for these? Here is my gel with delta12 and delta21 with the isolates of WLP644 and Conan that I used as the parent strains: Edit: Hybrid H1 = F1
  20. S

    Conan x WLP644 hybrid

    I'm hoping to grow up a new batch towards the end of May, but I can't promise anything :)
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