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I'd like to think you're right, if temps were really that much off, then there'd be a really low efficiency, which I have yet to hear about.

It sounds like you're thinking too much into this. Your yeast quit it seems to me. First try rousing them and if that doesn't work, then re-pitch. I could be wrong.

IPA brewed today and pitched on top of a Pale Ale yeast cake of US05. ~100 IBU of Cascade and Vic Secret. All Golden Promise and OG of 1.065. Second time brewing this and although I am overpitching, it worked before. Airlock started two hours after it went in the fermenter.

Thank you Vale, Neo and Passedpawn. You've given me a lot to think about. Very much appreciated.

Hi all, I'm new here and didn't find an answer on existing posts. Has anyone used methylene blue to check yeast viability? My understanding is that pro brewers use (or use to use) it to get a better handle on the number of live cells they're pitching. Or is it just not worth doing on a...