I am embarking on brewing an extract NEIPA about 1.084 OG. I am making a yeast starter but for an appropriate size starter, the volume of the vessels I have, and the age of my yeast, I am stepping it up. My first batch is 1.6 Liters, my second is going to be 3.5 Liters. I will skim between .5 and 1 Liter of the overall matrix when done to keep for later batches. My question is this: typically you would take the starter off the stir plate at about 18 to 24 hours (maybe longer) lets say, then let it rest for about 8-12 hours at temp to build up glycogen reserves to get ready to pitching to the wort. But if I am stepping up, do I need the extra rest 8-12 rest for the glycogen reserves or can I just refrigerate (flocculate), decant, and start the mother batch (3.5L) as soon as it flocculates (12-24 hours later) the next day? Any thoughts? This is my last extract brew before moving to all rain next month. I need about 500+ billion cells.