Brewing chemists attribute skunky flavor to 3-methylbut-2-ene-1-thiol, a constituent of skunk spray. This compound arises from a reaction that light triggers within the beer, and the resulting taste can overwhelm other flavors.
"Humans are very sensitive to this compound," says Denis De Keukeleire of Ghent University in Belgium. 3-methylbut-2-ene-1-thiol is detectable in quantities as small as 0.004 microgram (µg) per liter. This amount can form in minutes when beer is exposed to bright sunlight, he says.
Riboflavin, a compound produced by yeast during fermentation, absorbs energy from light at wavelengths of 350 to 500 nanometers (nm). It transfers the energy to iso-alpha acids, the compounds that give beer bitterness. They then release free radicals—small, unstable chemical fragments—that react with sulfur compounds produced by the yeast. The result is the offending thiol.