Simonh82
Well-Known Member
- Joined
- Dec 4, 2015
- Messages
- 85
- Reaction score
- 10
I have a small collection of yeast cultures that I keep on slants. Some of them are getting on for 6 months old and I'd like to restreak them onto fresh slants to keep the strain going. Is it fine to just transfer and streak directly from the old slant onto a new one? Will the viability of the yeast have deteriorated significantly during storage and will it grow new healthy cultures on the fresh slant, or will they have suffered from the long storage? As I'm planning on using the old slant for a brew, would it be better to grow that up in a small starter and take a slant from that, increasing the vitality of the cells being streaked? The obvious issue being that this would increase the risk of introducing contamination, even if it were at a very low level you might find in a starter that had been boiled for 15 minutes.
Any thoughts on the best way to proceed would be appreciated.
Any thoughts on the best way to proceed would be appreciated.