Proper Iodophor Concentration for Sterilization

Homebrew Talk - Beer, Wine, Mead, & Cider Brewing Discussion Forum

Help Support Homebrew Talk:

TheJadedDog

AFK ATM
Joined
Aug 27, 2006
Messages
3,310
Reaction score
17
Location
People's Republic of Cambridge
Okay, so I definitely have something unwelcome living in my primary and ruining my beers :mad: That said, does anyone know the correct sterilization concentration of iodophor for 6.5 gallons? I want to soak the carboy and try to kill off whatever is in there. Thoughts?
 

flyangler18

Well-Known Member
Joined
Jun 5, 2008
Messages
5,557
Reaction score
44
Location
Hanover, PA
My completely unscientific 'from the hip' answer would be half the normal dilution; however, if you have a bug that is ruining beers, go scorched earth with bleach.
 

donaldson

Supporting Member
HBT Supporter
Joined
Dec 1, 2007
Messages
19
Reaction score
0
Location
London, Ontario
Actually the directions on my bottle of iodaphor says 1ml per liter. That'd be about 4 ml/gallon and 5 ml is about 1 US teaspon. So multiply that by 5 and there yah go - 5 teaspoons - if you want a full soak that is. Seems like over kill. +1 on the bleach btw. I like to mix it up. Keeps the nasties guessing.
 

Hugh_Jass

Well-Known Member
Joined
Nov 23, 2008
Messages
1,956
Reaction score
44
Tanks for the response on the tsp. conversion.

Salute! :mug:
It also depends on the % of concentrate. Revvy uses 1%. I could only find .5% (thanks meth lab peoples). With .5%, I use three tsp/two gallon.
 

billtzk

Well-Known Member
Joined
Apr 5, 2007
Messages
1,617
Reaction score
23
Location
Dallas
Is this a glass carboy or a Better Bottle (plastic)? If glass and you are doing normal sanitizing (note: not "sterilization" as in your post title), then I'd look somewhere else for the source of the problem.

Carefully review all of your procedures, including your water source if you are topping up. You could be getting an infection from spoons or other stirrers or airlocks or bungs or hydrometers or thief or anything else that comes in contact with your wort.

Normal concentration of Iodophor for sanitizing is 12.5 PPM. That is 1/4 ounce (1.5 teaspoons) in 2.5 gallons, or 1/2 ounce (one tablespoon) in 5 gallons. You really don't need to use more than a half gallon to sanitize a carboy (1.5 ml to 1/2 gallon of water, or a little more than 1/4 teaspoon).
 

billtzk

Well-Known Member
Joined
Apr 5, 2007
Messages
1,617
Reaction score
23
Location
Dallas
Actually the directions on my bottle of iodaphor says 1ml per liter. That'd be about 4 ml/gallon and 5 ml is about 1 US teaspon. So multiply that by 5 and there yah go - 5 teaspoons - if you want a full soak that is. Seems like over kill. +1 on the bleach btw. I like to mix it up. Keeps the nasties guessing.
This really depends on the concentration of the stock solution. The amounts you give are too much if you are using B-T-F Iodophor. It should be 3 ml (1/10th US ounce) per gallon for 12.5 PPM. So for 5 gallons, 15 ml which is one tablespooon. That is three teaspoons, not five.
 

fratermus

Well-Known Member
Joined
Feb 18, 2008
Messages
1,188
Reaction score
3
Location
75081
Okay, so I definitely have something unwelcome living in my primary and ruining my beers :mad: That said, does anyone know the correct sterilization concentration of iodophor for 6.5 gallons? I want to soak the carboy and try to kill off whatever is in there. Thoughts?
I do not think there is a published sterilization rate for iodophor. I'd have to go back and list to that BB podcast to see if he says anything about sterilization.
 

Joe Camel

Well-Known Member
Joined
Mar 15, 2008
Messages
282
Reaction score
4
Location
Charlottetown, PE, Canada
If there is something living in your primary and is resisting your normal sanitizing techniques (2-5 min contact with iodophor?) then it's likely a cleaning/sanitizing issue and not a sanitizing issue alone and I'm not sure a long soak in iodophor will help.

Most sanitizers won't penetrate deep into caked on gunk and films so anything living within the gunk and film will be protected. So the issue may be more with the way you clean rather than the way you sanitize. How do you typically clean your primary?
 

BarleyWater

Well-Known Member
Joined
Jan 31, 2007
Messages
2,199
Reaction score
28
Location
Elmhurst
IIRC, Iodophor will not sterilize anything, only sanitize which may not be good enough for you. I think the best thing you could do, aside from backing it in an oven, would be a bleach soak. I Tablespoon of bleach, and 1 tablespoon of plain white vinegar makes a deadly mixture in 5 gallons of beer (DON'T MIX TOGETHER, mix into the water separately unless you want to asphyxiate). Let that soak a while and you may be good to go, but I still don't think you are going to get sterilization without heat. 350 for 3 hours will kill anything and everything and result in 100% sterilization.
 

johnafink

New Member
Joined
Aug 6, 2008
Messages
3
Reaction score
0
Sterilization (microbiology)
From Wikipedia, the free encyclopedia

Sterilization refers to any process that effectively kills or eliminates transmissible agents (such as fungi, bacteria, viruses, spore forms, etc.) from a surface, equipment, article of food or medication, or biological culture medium.[1][2] Sterilization does not, however, remove prions. Sterilization can be achieved through application of heat, chemicals, irradiation, high pressure or filtration.

Chlorine bleach is another accepted liquid sterilizing agent. Household bleach consists of 5.25% sodium hypochlorite. It is usually diluted to 1/10 immediately before use; however to kill Mycobacterium tuberculosis it should be diluted only 1/5, and 1/2.5 (1 part bleach and 1.5 parts water) to inactivate prions. The dilution factor must take into account the volume of any liquid waste that it is being used to sterilize.[11] Bleach will kill many organisms immediately, but for full sterilization it should be allowed to react for 20 minutes. Bleach will kill many, but not all spores. It is highly corrosive and may corrode even stainless steel surgical instruments.

Bleach decomposes over time when exposed to air, so fresh solutions should be made daily
 
Top