Perhaps a slight quality issue with SA, but after getting a case of Infinium to split among friends we discovered one had a good amount of sediment still on the bottom (I thought this would all be removed via their champagne "like" process). I was able to, with some sterility transfer it between 2X 50ml BD tubes, perhaps a total of 10mL of dregs were collected and split between 2 tubes...
Over the last 14 days I have been scaling up one of the tubes and today I hit approx 2.5 liters and 98 million or 9.86E^6cells according to my cell count via hemacytometer. My observations show very good viability and identity between the cells in the culture, tripan blue did not show too much cellular damage.
I was not able to find out too much on my own about what their process or yeast is but I am going to plate/slant a few samples for my collection. I feel pretty good about this! It was all very tasty!
Before and After:
http://img59.imageshack.us/i/img0537small.jpg/
Over the last 14 days I have been scaling up one of the tubes and today I hit approx 2.5 liters and 98 million or 9.86E^6cells according to my cell count via hemacytometer. My observations show very good viability and identity between the cells in the culture, tripan blue did not show too much cellular damage.
I was not able to find out too much on my own about what their process or yeast is but I am going to plate/slant a few samples for my collection. I feel pretty good about this! It was all very tasty!
Before and After:
http://img59.imageshack.us/i/img0537small.jpg/