Frozen yeast

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HairyDogBrewing

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I harvested and washed the yeast (WLP001) from a blonde ale last Friday.
I left the refrigerator door ajar this morning and everything that was touching the wall is frozen, including 2 of the 3 jars of yeast. :(
A can of Coke exploded too.
My understanding is that ice crystals can puncture the cell walls and kill them, unless you use glycerin.

I'm willing to risk some DME on a starter, unless there's zero chance of survival.
Has anyone successfully revived yeast slurry that has been frozen without glycerin?
 

Professor Frink

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That's a tough call. I would try to grow them up with some DME, depending on how cold it got, it's possible not all of the yeast froze.
 

mysteryberto

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I doubt anything will survive as freezing will rupture the cell walls when it comes back to normal temperature. I would chock it up as a loss considering how common WLP001 is. Still it might be interesting to see if anything would come back to life.
 
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