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So i lied... there are viable cells, after 2 days I added the rest just to see what will happen and it grew and fermented, had a nice krausen. But the viability is probably pretty low. I'll try with some glycerol this weekend though.

Alright, sorry this took a while to respond to... I think the lyophilization killed the yeast, I'll try another time with some glycerol, I wanted to give it some extra time to see if they would start growing but as of now I don't really see any activity.

got the yeast powder, it has some yeast extract and tryptopne, i stored it ina 2xty solution rather than pure water. So I will drop it into some sugar water and will see if i get any activity at all.

Yeah I was thinking about adding some glycerol, but I didn't this time around, this is just some sefal US-05 i decided to save, so nothing special.

sounds like a plan, I will probably lyophilize them tomorrow night, and hopefully I'll do a quick dirty test with sugar water, and if that works I will probably send them to you to test the viability

So I got another question... I have access to a lyophilizer, and liquid nitrogen, do you guys think lyophilizing yeast will be a good idea? I head that it's possible to lyophilize and then just store in a fridge while it's dry for years.... Also this will make sending them out easier, cuz you...

Also, another thing i should mention, although slanting and washing of the yeast is fine, another alternative, which might be even better, is to save them in a glycerol "freezer stock" which will remain viable for a much longer period of time, and which only requires that you scrape some of the...

Hey all, So I have some Petri dishes with yeast and some culture tubes with yeast and I'm not entirely sure how to fill out this information out. Some of the yeast are straight out of the bag, i.e. I had some left over in the bag post pitching and I just diluted it down with some clean water and...

+8 = 23002.5

Wood