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  1. WoodlandBrew

    Simple Yeast Storage Procedure

    Simple Yeast Storage Procedure 1) Sanitize or sterilize jars. (Mason jars work nicely for this purpose. A pint size jar which will hold about 300 billion cells) 2) Leave behind about an inch of beer when racking the beer off the yeast cake. 3) Swirl the fermentor to suspend the yeast. (Avoid...
  2. WoodlandBrew

    Simple Yeast Storage Procedure with Photos

    Simple Yeast Storage Procedure 1) Sanitize or sterilize jars. (Mason jars work nicely for this purpose. A pint size jar which will hold about 300 billion cells) 2) Leave behind about an inch of beer when racking the beer off the yeast cake. 3) Swirl the fermentor to suspend the yeast. (Avoid...
  3. WoodlandBrew

    http://www.homebrewtalk.com/showthread.php?t=579350

    Simple Yeast Storage Procedure 1) Sanitize or sterilize jars. (Mason jars work nicely for this purpose. A pint size jar which will hold about 300 billion cells) 2) Leave behind about an inch of beer when racking the beer off the yeast cake. 3) Swirl the fermentor to suspend the yeast. (Avoid...
  4. WoodlandBrew

    What size flask?

    Depending on the size of your beer, the starter volume will vary. The question here is what size do you normally end up making for your beers? Based on that, what size would you find most useful?
  5. WoodlandBrew

    WBR: Automated Viability

    I've been doing some work with microscopy and viability and wanted to share what I have learned. Whether or not you are familiar with ImageJ or automated image analysis you might find this interesting. http://www.woodlandbrew.com/2015/02/automated-cell-counting-of-yeast-using.html
  6. WoodlandBrew

    WBR: Starter Calculator showing cell growth over time

    How time effects cell growth is a factor that I have felt is missing in the existing calculators, which led me to create this starer calculator. I've collected data from dozens of experiments and factored it all down into this Excel calculator. This calculator also lets you select which...
  7. WoodlandBrew

    CellProfiler and ImageJ

    What are people using these days to automate yeast cell counts? I'm collecting several hundred images for an experiment that I am running and am looking record viability, cell size, and hopeful be able to identify abnormally shaped cells. I've been doing it manually, which isn't too bad, but...
  8. WoodlandBrew

    WBR: Cell Density Meter Update

    Here's how our Cell Density Meter is coming along, and a study using it to compare yeast starter growth! http://www.woodlandbrew.com/2015/01/cell-density-meter-update.html
  9. WoodlandBrew

    Fermentation Performance of Refrigerated Yeast Slurry

    There seems to be room for research on the effects of refrigeration on yeast slurry. There are online calculators that will produce a viability number based on storage time , but the research behind these calculations seems to be lacking.[1] Work has been done examining the effects over the...
  10. WoodlandBrew

    Yeast Cell Density Meter

    Do you want a tool that can measure the amount of yeast in your starter or wort, and is easy to use? A product development team that I'm excited to be part of has started design of a beer color and cell density meter. What would you expect in such a device? How about easier to use...
  11. WoodlandBrew

    My Stir Plate Works Better as a Blender

    My DIY, computer fan and hard drive magnet stir plate spins a vortex than would make a typhoon jealous, but it's performance when it comes to propagating yeast is less than stellar. In a side by side test an un-agitated starter outperformed this stir plate. The first stir bar I used was a...
  12. WoodlandBrew

    Practicality of Methylene Blue Staining

    Last night I was reading a paper discussing viability assays by Thiele and Back thanks to my wife who works at a university. It had one of the best descriptions of the advantages and disadvantages to Methylene Blue staining that I have ever read, and it is much better presented than I could...
  13. WoodlandBrew

    Yeast - 6

    I'm looking to explore some other methods of testing viability of yeast. Is any one familiar with the slide culture method? Or even better, have experience with the Yeast - 6 method as described by the American Society of Brewing Chemists? (Or if anyone has a copy of it that would be...
  14. WoodlandBrew

    Does Fruit Lower the Viability of Yeast?

    Has anyone else seen the addition of fruit lower viability? In all three of the most recent fruit beers I have made the viability of the yeast post fermentation was very low. In one case I measured the viability of the suspended yeast before adding the fruit and it was over 90%, but the...
  15. WoodlandBrew

    Any idea what this microbe might be?

    Any one know what this cell might be? It is rod shaped. About 5 microns in diameter and about 15 micron long. It looks kind of like Streptobacilli, but it is much larger. I have read that wild yeast look like this, but never seen any. This is in an experimental wort in my kitchen that I...
  16. WoodlandBrew

    Lab testing for slurry cell counts and viability

    Is there interest in knowing the cell count of slurries? It’s something that I find particularly useful especially since discovering how wide the viable cell count can range. A variation of a factor of ten is more common than many brewers would be comfortable with. If a sample of...
  17. WoodlandBrew

    Cold Crashing and Bottling

    I've had good results cold crashing for two days in a refrigerator before bottling. There is another technique that is similar that seems to be popular that I am curious about: 1) Cold crash the beer for a week or longer 2) When racking the beer to the bottling bucket, stir up the cake to...
  18. WoodlandBrew

    Help selecting a hemocytometer and microscope

    For counting yeast cell density and viability what would be the most appropriate tools? With a few searches on this site it seems that the $20 Hemocytometer, and an inexpensive microscope with 400x magnification are adequate, but I would like to hear about peoples experience with cell...
  19. WoodlandBrew

    How big of a starter?

    It seems that both Mr. Malty and YeastCalc base their growth rates and cell counts on data produced by the same experiment at White Labs. Does anyone know of another source of data correlating starter size to yeast populations? Perhaps experiments they have done themselves? Last weekend...
  20. WoodlandBrew

    Raspberry Cream Ale - Looking for feedback

    My wife loved the Raspberry Wheat beer we made recently, but the temperatures here have dropped a little too far for the Saision yeast that we used for that beer. We're not quite down to lagering temperatures yet, so I thought a cream ale might be worth a try. I would love to hear some...
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