I get worried about infections mostly when I'm twinking with slants and plates. This took a lot of worry out of it, at least for me. I'm kinda anal....
LOL! I don't know why but i thought this was going to have something to do with the glove box of a car!
Sorry, but you're in the "UBER-ANAL" section now...
Wow. Just, wow. I've never seen anything like that in the homebrew setting. Jamil Z. will be jealous (if he doesn't have one already).
You could also use it to bathe your cat.
You can use an alcohol burner inside, but chemical sterilants such as Lysol are more commonly used.
What's your ventilation system like on this?
That's pretty creative and I love the toilet flanges. However, how can you culture yeast with sterile technique using that? I understand it isolates dust particles but how are you maintaing sterility of your equipment in there by not being able to flame?
You could also use it to bathe your cat.
The box is sanitized by spaying inside with a disinfectant on all surfaces and gloves 30 min before use. All equiptment is sterilized beforehand in a pressure cooker at 15lb for 30 min, then unwrapped inside the box. I do use a flame for the needle and loupe, although even as anal as I am, it is probably overkill..
Well if you are working with multiple cultures I don't see that the flame is overkill unless you have separate inoculation loops for every culture you are doing.
On short notice tonight I made 5 plates, stepped up 2 starters from 10ml to 100ml, and made 2 2L starters. All around my little alcohol burner on my clothes washer.
As cool as I think it would be to have something like this I think it would just increase the time it takes to do everything.
That thing is BAD!!!!! I love it! I want one.
If you aren't filtering the air in the box how is it anymore sterile than your countertop? Manipulating your samples inside the glovebag, as you mentioned, is slower that doing it with unencumbered hands. The key for a success in microbiology is quick and accurate procedures that minimize the opportunity for contaminination. For delicate samples a laminar flow hood is the proper tool.
A glovebag would be a much simpler DIY solution.
Just look at how happy this biology student is
Large clear plastic bag, kitchen gloves, duct tape, and an air compressor hooked to a hepa filter to pressurize the bag.
Just some thoughts, nice work on the box.
Thanks, WM. I'm doing all my starters. dishes and slants in it now. we'll see how it turns out...That thing is BAD!!!!! I love it! I want one.
A glovebag would be a much simpler DIY solution.
Just look at how happy this biology student is
The box is filtered through a hepa filter as linked above. I used the plastic bag idea for a while, but it was a PITA. Have ypu priced a laminar flow hood?
The box!!! That thing rocks!:rockin:Are you talking about the glove box or the biology student er I mean the glove bag?
The box!!! That thing rocks!:rockin:
6 years....
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