Made a 2.5L starter of WLP002 yesterday, no stir plate so using the swirl method, been swirling about 5 times an hour if not more. I want to chill it then decant liquid and step this up into a 3L starter. How do I know that this 2.5L starter is read so I don't waste any yeast when I decant off the liquid after cold crashing. Plan on using it in a RIS with a target OG of 1.106. When I swirl the starter now a nice white foam about an inch thick rises then soon dissipates but I can also see the CO2 bubbles in the wort also. The plan was to cold crash tomm morning giving it 36hrs of "working time" at that point. Will it be ready or should I wait longer. Plan on brewing Thursday and I still have to make the other step up starter. Thanks for the help.