I want to know what makes more cells,
making several small starters, or creating one large one.
I want to pitch about 1-1.5 liters for my next beer. say i make 750 ML starters and use 250 ML as my yeast source for the next starter. so in 3 days of creating starters, i have 1.5 liters of yeast starter ready to go
should i make a single 1.5 liter starter?
to me it seems like a single vial of yeast would have trouble propagating to the same number of cells in a 24 hour period, as it would in 3 days (3 successive starters) even if they had a larger original volume (breathing room) for the initial 24 hours
i mean, do yeast really worry about what concentration of cells there is in a given volume, or do they just propagate like crazy in the first 24 hours (or so) and then switch to anaerobic production.
If they dont care about volume then i know the answer:
for instance (and these numbers are not scientific)
If yeast can only reproduce a certain number of times in a given amount of time you can introduce 100b cells (a vial) into a starter (volume does not matter), say yeast can double their cell count in 24 hours. so now in 1 day you have 200B. and then you do it again and again and end up with mindboggling ammounts of cells
small batches sound like they are the way to go. BUT, if they do care about volume, then i am not sure. (ie, do they get more efficient at repoduction if they have more breathing room)
100B cells into 1L might make 200B cells in 24 hours, but might make 400B in a 2L starter just because the yeast are more comfortable.
anyone know an answer?