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Old 08-18-2013, 01:35 AM   #1
FredTheNuke
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Feb 2012
Charlotte, NC
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What is the longest anyone has attempted? I just started waking up 3 vials of May 2012 slanted Wyeast Urquell Pilsner yeast... Each is in 250ml of fresh wort on stir plates... Tomorrow will tell.
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Old 08-19-2013, 12:28 AM   #2
FredTheNuke
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Feb 2012
Charlotte, NC
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No bueno thus far. S.G. still at 1.036 from 1.040. 24 more hours just to be sure and then time to hit the eject button.

 
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Old 08-19-2013, 11:15 AM   #3
Mb2658
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Nov 2011
, NE
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It will probably take a few days. I typically start at 50 to 100 mL on my first step and that can take a day or two. That is using frozen yeast in 4 dram vials.

 
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Old 08-19-2013, 12:59 PM   #4
FredTheNuke
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Feb 2012
Charlotte, NC
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bingo! This morning (36 hours) I was starting to get chocolate milk instead of straight wort. Too bad one of my 3 stirplates died overnight... New one on order but will have to collapse down to 2 starters (I have until Saturday to grow them big enough for 30 gallons of lager - gonna be a stretch but I am growing up to 5000ml flask size).

 
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Old 08-21-2013, 03:02 PM   #5
FredTheNuke
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Feb 2012
Charlotte, NC
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Update for anyone wondering if a 15 month old slant (kept at 36 degrees the entire time) will come alive. I stepped up to 3500ml per starter last night and they are offgassing so fast you see continuous CO2 bubbles - very active. All 3 look and smell the same (most likely the actual Sacc yeast is what took hold not an infection). Slanting is an amazing technique!!!

 
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Old 08-23-2013, 01:52 AM   #6
wegz15
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Oct 2009
Northbrook, Illinois
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I've used slants from september 2011. I start at about 100mL for about 2 days then step up to 2L. I also count my yeast. I've read somewhere on here to step in 1:10 or 1:100 intervals (100mL pitched into 1L or 10mL to 1L) can't remember. I'll try to find it.

 
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Old 08-30-2013, 04:01 PM   #7
PaintedRat
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Feb 2012
Fresno, CA
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I'm 1 for 2 in reviving two year old vials from a friend's fridge. Procrastination was the only thing that granted the start the extra day to show visible signs. Old slant to starter for a 30gal batch is pretty awesome.
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Old 08-31-2013, 10:52 PM   #8
FredTheNuke
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Feb 2012
Charlotte, NC
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It has a 1.059 OG down to 1.026 after 6 days at 52F. Chugging along but much slower than the first few days.

 
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