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Old 10-18-2007, 10:12 PM   #1
c.n.budz
 
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I picked up a growler of Dead Guy Ale and a bottle of Rochefort that I plan on drinking tonight and want to harvest the yeast but won't have a chance to do that until either tomorrow night or saturday morning. Can I just re-cap both bottles and stick them in the fridge for a day or two?
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Old 10-18-2007, 10:31 PM   #2
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I'm not sure if that would be a problem or not - you're trying to be as sanitary as possible when culturing from a bottle b/c of the small amount of yeast and their potential low viability.
But either way make sure your starter is very small initially. The first time I tried to culture Roch was in ~500mL starter and that is tooo big - didn't work. Start small and work up -you probably already knew that. Good luck.

 
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Old 10-18-2007, 10:34 PM   #3
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You never want to exceed 5 times the volume of the voulme you are culturing. So if you get 10ml, use a starter of around 25-50ml, after 8 hours go to 100ml, etc.

 
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Old 10-18-2007, 10:44 PM   #4
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I'm not looking to make a starter out of them yet, just want to harvest the yeast. So my main concern is if I need to transfer the small amount of yeast from the bottles into a smaller sanitized vessel tonight or if I can just store it in the beer bottles for a day or two in the fridge before transferring it for longer cold storage
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Old 10-19-2007, 01:34 AM   #5
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A day or two will probably work, but I would get to it as soon as possible. What media are you planning to use to store the yeast?

 
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Old 10-19-2007, 01:48 AM   #6
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Quote:
Originally Posted by Iordz
What media are you planning to use to store the yeast?

My roommate is a glassblower for a scientific glass company, he made me a bunch of small glass jars with airtight seals
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Old 10-19-2007, 01:16 PM   #7
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IMHO, I would not store the yeast from the bottles without first making a small starter with it to help increase its viability. If it's already quite weak, you're probably not helping it any by leaving it in near-empty bottles for a while and then transferring it around, without giving it any food to let it build up its strength again.

 
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Old 10-19-2007, 01:25 PM   #8
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Quote:
Originally Posted by Funkenjäger
IMHO, I would not store the yeast from the bottles without first making a small starter with it to help increase its viability. If it's already quite weak, you're probably not helping it any by leaving it in near-empty bottles for a while and then transferring it around, without giving it any food to let it build up its strength again.
I've got to second this; I'm not sure how successfully you can culture from a bottle without making some kind of small starter.
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Old 10-19-2007, 03:45 PM   #9
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so these two beers were not filtered at all, right?
and the growlers were sanitized before adding the beer...you're 100% certain?
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Old 10-19-2007, 03:48 PM   #10
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You're better off posting around to see who has the Pacman yeast still and see if you can't get them to send you some.
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