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Old 10-07-2007, 09:32 PM   #1
CodeRage
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Hey guys, I had that wyeast pack to store and I was bored so figured I would try something. I cooked up some home made agar. 1 Cup of water, 1/3 cup of DME, 1/8 tsp of yeast nutrient, and one packet of gelatin. Poured it into 3 'contemporary' mason jars (had enough for 4 actually). Its all cooling now. Probably will innoculate them in the next hour or so. What do you all think?

 
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Old 10-07-2007, 10:05 PM   #2
mayday1019
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Jan 2006
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Pardon my ignorance, but what is agar??

 
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Old 10-08-2007, 02:09 AM   #3
CodeRage
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Its kind of like a nutrient rich jello you can culture bacteria, mold, and fungus on.

 
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Old 10-08-2007, 02:12 AM   #4
sudbuster
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Quote:
Originally Posted by Virtuous
Hey guys, I had that wyeast pack to store and I was bored so figured I would try something. I cooked up some home made agar. 1 Cup of water, 1/3 cup of DME, 1/8 tsp of yeast nutrient, and one packet of gelatin. Poured it into 3 'contemporary' mason jars (had enough for 4 actually). Its all cooling now. Probably will innoculate them in the next hour or so. What do you all think?
Ahmmm. Sorry, no comment.....

 
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Old 10-08-2007, 02:56 AM   #5
CodeRage
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Quote:
Originally Posted by OldFarmer
Ahmmm. Sorry, no comment.....
You're just as useless as tits on a boar j/k

 
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Old 10-08-2007, 02:41 PM   #6
pjj2ba
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It should work fine. It is basically what I do. I weigh out my DME (60 gms/liter) dissolve that and then add 20 gms of agar (2% final conc. wt/vol). Slap it in the autoclave and pour into pertri plates when cool enough. The yeast grow fine.

 
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Old 10-08-2007, 03:28 PM   #7
cge0
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Aug 2007
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Yeah, autoclaving is a must. You can use a pressure cooker, just leave it in there for 60-90 min. Make sure your technique is sterile.

 
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