depends on how old the vial is. I prefer to try to double my growth each step, or 50 to 75 mill/ml (innoculation rate).
example: I brew 12 G batchs, so if my viability of a vial is 70%, that is 70 bill cells instead of 100 bill cells per vial.
I split the vial up front and do two starters simultaneously, effectively putting 35 bill cells per starter. I will make 2x .5L , decant, and make 2x .8L starter for brew day. I would not see much more growth if I made the first step 1L vs a 2L. which is why you want to keep an eye on the innoculation rate. You end up saving DME or starter wort compared to what you would go through otherwise
Originally Posted by Common N00B
I just got a Mr Beer kit and want to brew a Double Imperial Blueberry Heffy Witesit....we have no air conditioning and live next to the sun...do you think I can logger and bottle this in time for a party I will be having next week?