I need some help from fellow yeast washers...
I recently made a starter with some washed WLP838 that I had from a Dunkel.
I would guess that I had about 1oz of yeast (some sediment and trub mixed in) in the mason jar that I used.
My first step was to take this washed yeast and add it to 2L of 1.040 wort on the stirplate for about a day. After being on the stirplate, I cold crashed the 1 gallon jug I am using for a little over a day. I had a small amount of yeast on the bottom but not a ton so I stepped the starter up with another 2L of 1.040 wort after decanting out as much of the beer from the jug as I could.
Now it has been about 36 hours later and I have had the jug cold crashing for a few hours and noticed what seems to be a good amount of yeast on the bottom (see picture).
My question is this... How can I know how many yeast cells I started with in my washed mason jar (washed at the end of September 2012) and also, does it look like I have enough in this 1 gallon jug to add to 8.5 gallons of wort to make a Helles this weekend?
I hate making starters, especially stepping them up if I don't have to so if I have enough yeast now, I would like to call it a day and get started brewing.
I have used the calculator on yeastcalc.com but its kind of pointless not knowing my starting yeast counts. Can anyone please help me out with this?
Thanks in advance!