National Homebrew Day Giveaway - Enter Now - Weekend Giveaway!

Home Brew Forums > Home Brewing Beer > General Techniques > Guide to Making a Frozen Yeast Bank
Reply
 
Thread Tools
Old 01-31-2012, 09:56 AM   #601
emjay
HBT_SUPPORTER.png
Feedback Score: 1 reviews
 
emjay's Avatar
Recipes 
 
Join Date: Jan 2011
Location: Toronto, Ontario
Posts: 12,712
Liked 1715 Times on 1603 Posts
Likes Given: 1

Default

Quote:
Originally Posted by Nightbiker
Thanks! I'll give 'em a look-see. I'm assuming that borosilicate glass (it says that the cap is even autoclavable -which is excellent) is also freezer safe?
Yes...
emjay is offline
 
Reply With Quote
Old 01-31-2012, 01:10 PM   #602
Zamial
Feedback Score: 0 reviews
Recipes 
 
Join Date: Apr 2010
Location: WI
Posts: 3,184
Liked 169 Times on 150 Posts
Likes Given: 7

Default

This may be helpful...

I recently bought 4 of these: http://www.midwayusa.com/product/156...-round-plastic in the smoke gray to hold the Cynmar 16ml vials.

Without any modding these are worthless. The vials can be forced into them but when the temp drops they are impossible to remove. The reason for that is inside each square is a tapered ridge, I assume it is used to keep the shotgun shell snug.

A quick trip to the craft store (I went to Hobby Lobby) and I bought a mixed pack of Exacto knife blades that had a thin, flat scrapper style blade. It was tedious but I scraped everyone of the ridges off and now the holder/box works like a charm. It was not hard to do but very tedious and a little messy with all the plastic bits.

Now they are all together and MUCH harder to knock over. Each box holds 25 vials I normally make 10-12 vials of each strain I bank so it works out well for me.
__________________
I'm not drunk, I'm from Wisconsin.
We have been out drinking your state since 1848!
Zamial is offline
 
Reply With Quote
Old 02-02-2012, 03:51 AM   #603
postal_penguin
Feedback Score: 0 reviews
Recipes 
 
Join Date: Sep 2011
Location: Aurora, Co
Posts: 173
Liked 4 Times on 4 Posts

Default

Quote:
Originally Posted by Nightbiker View Post
Uhm, I know I missed it, but where are you getting those vials? I'd love to have a batch of them -would really prefer larger ones (16 is such a small amount) -I've BEEN using sterile sample containers like we use in the hospital (110ml). I've never tried to harvest from a commercial bottle -and didn't know Bells bottle-conditioned. I love their brown ale -had it out of state once. Will have to see if I can find it and harvest some.
-thanks. Those vials look GREAT (love the tan look of yeast!)
Try out these: http://www.amazon.com/gp/product/B00...00_i00_details

15mL conical centrifuge tubes pack of 100 for $16. They are autoclavable and can handle -80C temps fine, I use them in the lab all the time. If you really want you can wash and re-use them.
__________________
Primary: Apfelwein, Raspberry Lambic, Experimental Wheat cider, IPA
Secondary: Empty
Keg 1: Hefe
Keg 2: Irish Stout
Keg 3: Peach Kolsch
Keg 4: Empty
Bottled: Bourbon Vanilla Porter, Chocolate Stout
postal_penguin is offline
 
Reply With Quote
Old 02-02-2012, 08:34 AM   #604
Nightbiker
HBT_SUPPORTER.png
Feedback Score: 0 reviews
 
Nightbiker's Avatar
Recipes 
 
Join Date: Jul 2008
Location: Dover, FL
Posts: 563
Liked 3 Times on 3 Posts
Likes Given: 5

Default

Thanks Postal -those look like a really nice deal. I did find a container at the hospital (where I work) -they are sterile sample containers -measuring 110ml with a locking, sealling cap -made of plastic -and I have a couple of yeast samples freezing as we speak. They cost more than the tubes from Amazon tho (about a quarter per unit -but then again, they are also quite a bit larger) -I'll keep using the larger sample container, but I think it would be smart to duplicate my efforts with the vials you listed (in a different freezer -just to be safe. Probably more effort than it's worth, but you know how it is ) We lost our big stand-up deep freezer -and along with it, all of my fresh frozen home-grown hops (cascades and mt. hood for the most part) -didn't notice it until it had completely defrosted, and the hops had turned a nasty shade of brown, and I won't even bother describing the status of my stockpile of venison and beefsteak.
Needless to say, I had to have quite a few consolation homebrews to get past that
__________________
GOT BEER?
FERMENTING:
LAGERING/CONDITIONING:Simply Sweet Mead
Kegged: Big Bad Brown Ale (Brown Ale and Porter blend).
Bottled:

Plans: Oak-Smoked Porter, Honey Brown Ale.
Quote:
Originally Posted by Jaybird View Post
we do it for the love of beer! Not for the love of money! We can all make great BEER! Not so much when it comes to money!
Nightbiker is offline
 
Reply With Quote
Old 02-02-2012, 12:27 PM   #605
mthompson
Feedback Score: 1 reviews
Recipes 
 
Join Date: Sep 2008
Location: Plymouth, WI
Posts: 530
Liked 45 Times on 28 Posts
Likes Given: 2

Default

Quote:
Originally Posted by postal_penguin

Try out these: Video Link: http://www.amazon.com/gp/product/B005Z4QSOS/ref=oh_o01_s00_i00_details

15mL conical centrifuge tubes pack of 100 for $16. They are autoclavable and can handle -80C temps fine, I use them in the lab all the time. If you really want you can wash and re-use them.
I thought about using those, but I've seen a lot of those plastic centrifuge tubes melt or deform in an autoclave.
__________________
MT
------------------------------------------------------------------------------------------
Save me, O God! For the waters have come up to my neck. - Psalm 69:1
mthompson is offline
 
Reply With Quote
Old 02-02-2012, 12:28 PM   #606
liebertron
Feedback Score: 0 reviews
Recipes 
 
Join Date: Feb 2011
Location: bowie, md
Posts: 83
Liked 2 Times on 2 Posts

Default

I'm not sure why people freeze the yeast. The liquid yeast isn't frozen when you get it and has a shelf life of a few months. Seems like with some planning you could just use one strain of yeast for several beers in a row within a couple of months...? Are people saving the yeast for long periods of time?
liebertron is offline
 
Reply With Quote
Old 02-02-2012, 12:37 PM   #607
bja
Feedback Score: 0 reviews
Recipes 
 
Join Date: Nov 2007
Location: Pittsburgh
Posts: 1,956
Liked 142 Times on 106 Posts

Default

Quote:
Originally Posted by postal_penguin View Post
Try out these: http://www.amazon.com/gp/product/B00...00_i00_details

15mL conical centrifuge tubes pack of 100 for $16. They are autoclavable and can handle -80C temps fine, I use them in the lab all the time. If you really want you can wash and re-use them.
What kind of gasket is used in the caps?
__________________
____________________________________________

I know it just wouldn't be right to kill all the stupid people that we meet, but do you think it would be appropriate to just remove all of the warning labels and let nature take its course?

Life is tough. It's tougher if you're stupid.
bja is offline
 
Reply With Quote
Old 02-02-2012, 12:57 PM   #608
mthompson
Feedback Score: 1 reviews
Recipes 
 
Join Date: Sep 2008
Location: Plymouth, WI
Posts: 530
Liked 45 Times on 28 Posts
Likes Given: 2

Default

Quote:
Originally Posted by liebertron
I'm not sure why people freeze the yeast. The liquid yeast isn't frozen when you get it and has a shelf life of a few months. Seems like with some planning you could just use one strain of yeast for several beers in a row within a couple of months...? Are people saving the yeast for long periods of time?
Some of the yeast I have frozen, you cannot buy from the store. So, once I got it cultured up from the bottle I freeze a whole bunch and can pull it out next year or later and make a starter.

Plus the viability of yeast simply washed and stored in the fridge is not good at all.... Maybe for 2 weeks, after that you definitely need a starter anyway. The yeast you buy from white labs is not even close to the same quality that you have coming out of your fermentor. Dead yeast, hops, alcohol and bacteria all deteriorate a washed yeast slurry very quick.

I do wash yeast, and try to plan it out so I am brewing the same day and pitch a good healthy yeast. But if it doesn't work out, I know I can make a starter from the greatly more sanitary vials I have in the freezer. Not to mention you can buy a vial of American ale yeast, let's say, grow up a starter in sterilized wort then pull out a couple mL for freezing and pitch the rest. If you save 5 vials, you just cut your $7.50 yeast cost into 6 batches... So it saves money in the long run. The equipment is extra, and you can buy a lot of yeast for the money, but a lot of us have the stuff already.

With some extra equipment and little planning, I think I have bought two vials of white labs in the past few years. If I had not done this, I'd have spent $150 just on yeast alone. I had all the equipment, and now I most of my batches are under $20, $25 for this big ones.

It's not for everyone and it is definitely not necessary, but it works for a lot of us.
__________________
MT
------------------------------------------------------------------------------------------
Save me, O God! For the waters have come up to my neck. - Psalm 69:1
mthompson is offline
 
Reply With Quote
Old 02-02-2012, 01:00 PM   #609
mthompson
Feedback Score: 1 reviews
Recipes 
 
Join Date: Sep 2008
Location: Plymouth, WI
Posts: 530
Liked 45 Times on 28 Posts
Likes Given: 2

Default

Quote:
Originally Posted by bja

What kind of gasket is used in the caps?
No gaskets, they are plastic on plastic and meant to be disposable.
__________________
MT
------------------------------------------------------------------------------------------
Save me, O God! For the waters have come up to my neck. - Psalm 69:1
mthompson is offline
 
Reply With Quote
Old 02-02-2012, 04:54 PM   #610
Zamial
Feedback Score: 0 reviews
Recipes 
 
Join Date: Apr 2010
Location: WI
Posts: 3,184
Liked 169 Times on 150 Posts
Likes Given: 7

Default

I get mine here: http://www.cynmar.com/ProductDetail/...Cap-16ml--12pk they are completely reusable and autoclavable including the caps.
__________________
I'm not drunk, I'm from Wisconsin.
We have been out drinking your state since 1848!
Zamial is offline
 
Reply With Quote
Reply


Thread Tools


Similar Threads
Thread Thread Starter Forum Replies Last Post
Frozen Yeast Bank vs. Yeast Slanting shortyjacobs Fermentation & Yeast 11 09-24-2010 07:13 PM
Frozen yeast bank not freezing T-Hops General Techniques 6 03-17-2010 03:20 PM
Yeast Starter from frozen yeast bank dfohio General Techniques 5 04-30-2009 07:32 PM
Frozen Yeast Bank- Strain Exchange? flyangler18 General Techniques 37 03-25-2009 01:17 PM
Frozen yeast bank with yeast cakes? mrfocus Recipes/Ingredients 5 11-08-2007 01:13 AM


Forum Jump

Newest Threads

LATEST SPONSOR DEALS